Cyclin D2 ELISA Kit

Produktnummer ABIN5653415

Produktdetails Cyclin D2 ELISA Kit

Target: Cyclin D2 (CCND2)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.62 pg/mL - 1000 pg/mL

Untere Nachweisgrenze: 15.62 pg/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Cyclin D2 (CCND2). No significant cross-reactivity or interference between Cyclin D2 (CCND2) and analogues was observed.

Sensitivität: 5.9 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cyclin D2 (CCND2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cyclin D2 (CCND2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cyclin D2 (CCND2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cyclin D2 (CCND2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cyclin D2 (CCND2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cyclin D2 (CCND2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu Cyclin D2

Target: Cyclin D2 (CCND2)

Andere Bezeichnung: Cyclin D2 (CCND2 Produkte)

Synonyme: si:ch211-240j22.2 ELISA Kit, ccnd2 ELISA Kit, CCND2 ELISA Kit, kiak0002 ELISA Kit, zgc:162280 ELISA Kit, LOC100224096 ELISA Kit, KIAK0002 ELISA Kit, BF642806 ELISA Kit, C86853 ELISA Kit, Vin-1 ELISA Kit, Vin1 ELISA Kit, cD2 ELISA Kit, 2600016F06Rik ELISA Kit, AI256817 ELISA Kit, cyclin D2, b ELISA Kit, cyclin D2 S homeolog ELISA Kit, cyclin D2 ELISA Kit, G1/S-specific cyclin-D2 ELISA Kit, cyclin D2, a ELISA Kit, cyclin D2 L homeolog ELISA Kit, ccnd2b ELISA Kit, ccnd2.S ELISA Kit, CCND2 ELISA Kit, ccnd2 ELISA Kit, ccnd2a ELISA Kit, Ccnd2 ELISA Kit, ccnd2.L ELISA Kit

Hintergrund:

Gene Name: Cyclin D2

Gene Aliases: CCN-D2, G1/S-Specific Cyclin-D2

Gen-ID: 894

UniProt: P30279

Pathways: Zellzyklus, Mitotic G1-G1/S Phases