CrkL ELISA Kit

Produktnummer ABIN5653373

Produktdetails CrkL ELISA Kit

Target: CrkL (CRKL) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Crk Like Protein (CRKL). No significant cross-reactivity or interference between Crk Like Protein (CRKL) and analogues was observed.

Sensitivität: 0.058 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Crk Like Protein (CRKL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Crk Like Protein (CRKL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Crk Like Protein (CRKL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Crk Like Protein (CRKL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Crk Like Protein (CRKL) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Crk Like Protein (CRKL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu CrkL

Target: CrkL (CRKL) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL))

Andere Bezeichnung: Crk Like Protein (CRKL Produkte)

Synonyme: CRKL ELISA Kit, wu:fa55g05 ELISA Kit, wu:fj60e01 ELISA Kit, zgc:63469 ELISA Kit, 1110025F07Rik ELISA Kit, AA589403 ELISA Kit, AI325100 ELISA Kit, Crkol ELISA Kit, CRK like proto-oncogene, adaptor protein ELISA Kit, v-crk avian sarcoma virus CT10 oncogene homolog-like ELISA Kit, CRKL ELISA Kit, crkl ELISA Kit, Crkl ELISA Kit

Hintergrund:

Gene Name: Crk Like Protein

Gene Aliases: V-Crk Sarcoma Virus CT10 Oncogene Homolog(avian)-Like

Gen-ID: 1399

UniProt: P46109

Pathways: Interferon-gamma Pathway, Neurotrophin Signalübertragung, Signaling of Hepatocyte Growth Factor Receptor