CrkL ELISA Kit

Produktnummer ABIN2043245

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Maus CrkL.

Kurzübersicht für CrkL ELISA Kit (ABIN2043245)

Target: CrkL (CRKL) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 78.12-5000 pg/mL

Applikation: ELISA

Produktdetails CrkL ELISA Kit

Untere Nachweisgrenze: 78.12 pg/mL

Sensitivität: < 33 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Crk Like Protein (CRKL) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Crk Like Protein (CRKL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Crk Like Protein (CRKL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Crk Like Protein (CRKL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Crk Like Protein (CRKL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu CrkL

Target: CrkL (CRKL) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL))

Andere Bezeichnung: Crk Like Protein (CRKL)

Pathways: Interferon-gamma Pathway, Neurotrophin Signalübertragung, Signaling of Hepatocyte Growth Factor Receptor