Complement Factor B ELISA Kit

Produktnummer ABIN5653265

Produktdetails Complement Factor B ELISA Kit

Target: Complement Factor B (CFB)

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 6.25 ng/mL - 400 ng/mL

Untere Nachweisgrenze: 6.25 ng/mL

Applikation: ELISA

Proben: Plasma, Serum

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Complement Factor B (CFB). No significant cross-reactivity or interference between Complement Factor B (CFB) and analogues was observed.

Sensitivität: 2.92 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Factor B (CFB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Factor B (CFB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Factor B (CFB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Complement Factor B (CFB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement Factor B (CFB) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Factor B (CFB) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu Complement Factor B

Target: Complement Factor B (CFB)

Andere Bezeichnung: Complement Factor B (CFB Produkte)

Synonyme: AHUS4 ELISA Kit, BF ELISA Kit, BFD ELISA Kit, CFAB ELISA Kit, FB ELISA Kit, FBI12 ELISA Kit, GBG ELISA Kit, H2-Bf ELISA Kit, PBF2 ELISA Kit, bf ELISA Kit, Bf-1 ELISA Kit, Brre-Bf ELISA Kit, zgc:114032 ELISA Kit, Bf ELISA Kit, Sp152 ELISA Kit, bfb-A ELISA Kit, Bf B ELISA Kit, LOC397725 ELISA Kit, bf/c2 ELISA Kit, CFB ELISA Kit, AI195813 ELISA Kit, AI255840 ELISA Kit, B ELISA Kit, C2 ELISA Kit, Fb ELISA Kit, Da1-24 ELISA Kit, complement factor B ELISA Kit, complement factor B S homeolog ELISA Kit, complement factor B L homeolog ELISA Kit, CFB ELISA Kit, cfb ELISA Kit, bf ELISA Kit, cfb.S ELISA Kit, cfb.L ELISA Kit, bf/c2 ELISA Kit, LOC100555981 ELISA Kit, Cfb ELISA Kit

Hintergrund:

Gene Name: Complement Factor B

Gene Aliases: CF-B, BF, BFD, CFAB, GBG, H2-Bf, PBF2, B-Factor, Properdin, Properdin factor B, C3/C5 convertase, Glycine-rich beta glycoprotein

Gen-ID: 294257

UniProt: G3V615

Pathways: Komplementsystem, Proton Transport, Ribonucleoside Biosynthetic Process