C3 ELISA Kit
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- Target Alle C3 ELISA Kits anzeigen
- C3 (Complement Component 3 (C3))
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Reaktivität
- Maus
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 3.12 ng/mL - 200 ng/mL
- Untere Nachweisgrenze
- 3.12 ng/mL
- Applikation
- ELISA
- Proben
- Plasma, Serum
- Analytische Methode
- Quantitative
- Spezifität
- This assay has high sensitivity and excellent specificity for detection of Complement Component 3 (C3). No significant cross-reactivity or interference between Complement Component 3 (C3) and analogues was observed.
- Sensitivität
- 1.39 ng/mL
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- Kommentare
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Testdauer
- 3 h
- Plattentyp
- Pre-coated
- Protokoll
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Component 3 (C3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Component 3 (C3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Component 3 (C3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Complement Component 3 (C3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Testpräzision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement Component 3 (C3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Component 3 (C3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Handhabung
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Haltbarkeit
- 4-8 months
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- Target Alle C3 ELISA Kits anzeigen
- C3 (Complement Component 3 (C3))
- Andere Bezeichnung
- Complement Component 3 (C3 Produkte)
- Synonyme
- AHUS5 ELISA Kit, ARMD9 ELISA Kit, ASP ELISA Kit, C3a ELISA Kit, C3b ELISA Kit, CPAMD1 ELISA Kit, AI255234 ELISA Kit, HSE-MSF ELISA Kit, Plp ELISA Kit, C3 ELISA Kit, C3-1 ELISA Kit, sb:cb26 ELISA Kit, si:dkey-76b14.4 ELISA Kit, wu:fi34b03 ELISA Kit, wu:fj86e11 ELISA Kit, C3d ELISA Kit, asp ELISA Kit, cpamd1 ELISA Kit, c3 ELISA Kit, complement C3 ELISA Kit, complement component 3 ELISA Kit, complement component c3a, duplicate 1 ELISA Kit, complement component 3 L homeolog ELISA Kit, complement component C3 ELISA Kit, complement C3-like ELISA Kit, C3 ELISA Kit, c3a.1 ELISA Kit, c3.L ELISA Kit, LOC443475 ELISA Kit, c3 ELISA Kit, c3-1 ELISA Kit, LOC100060539 ELISA Kit
- Hintergrund
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Gene Name: Complement Component 3
Gene Aliases: ASP, CPAMD1, Complement Protein C3, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, C3a anaphylatoxin, Acylation stimulating protein
- Gen-ID
- 12266
- UniProt
- P01027
- Pathways
- Komplementsystem, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling
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