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CD276 ELISA Kit

CD276 Reaktivität: Human Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Produktnummer ABIN5653012
  • Target Alle CD276 ELISA Kits anzeigen
    CD276
    Reaktivität
    • 4
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.312 ng/mL - 20 ng/mL
    Untere Nachweisgrenze
    0.312 ng/mL
    Applikation
    ELISA
    Proben
    Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Cluster Of Differentiation 276 (CD276). No significant cross-reactivity or interference between Cluster Of Differentiation 276 (CD276) and analogues was observed.
    Sensitivität
    0.122 ng/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cluster Of Differentiation 276 (CD276). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cluster Of Differentiation 276 (CD276). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cluster Of Differentiation 276 (CD276), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cluster Of Differentiation 276 (CD276) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cluster Of Differentiation 276 (CD276) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cluster Of Differentiation 276 (CD276) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle CD276 ELISA Kits anzeigen
    CD276
    Andere Bezeichnung
    Cluster Of Differentiation 276 (CD276 Produkte)
    Synonyme
    sc:d0645 ELISA Kit, zgc:158279 ELISA Kit, 6030411F23Rik ELISA Kit, AU016588 ELISA Kit, B7RP-2 ELISA Kit, B7h3 ELISA Kit, B7H3 ELISA Kit, 4Ig-B7-H3 ELISA Kit, B7-H3 ELISA Kit, CD276 molecule ELISA Kit, CD276 molecule S homeolog ELISA Kit, CD276 antigen ELISA Kit, Cd276 molecule ELISA Kit, CD276 ELISA Kit, cd276.S ELISA Kit, cd276 ELISA Kit, Cd276 ELISA Kit
    Hintergrund

    Gene Name: Cluster Of Differentiation 276

    Gene Aliases: B7-H3, B7H3, CD276 Molecule, B7 homolog 3, Costimulatory molecule

    Gen-ID
    80381
    UniProt
    Q5ZPR3
    Pathways
    Cancer Immune Checkpoints
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