CMA1 ELISA Kit

Produktnummer ABIN5652951

Produktdetails CMA1 ELISA Kit

Target: CMA1 (Chymase 1, Mast Cell (CMA1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.2 pg/mL - 2000 pg/mL

Untere Nachweisgrenze: 31.2 pg/mL

Applikation: ELISA

Proben: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Chymase 1, Mast Cell (CMA1). No significant cross-reactivity or interference between Chymase 1, Mast Cell (CMA1) and analogues was observed.

Sensitivität: 12.5 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chymase 1, Mast Cell (CMA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chymase 1, Mast Cell (CMA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chymase 1, Mast Cell (CMA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chymase 1, Mast Cell (CMA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chymase 1, Mast Cell (CMA1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chymase 1, Mast Cell (CMA1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu CMA1

Target: CMA1 (Chymase 1, Mast Cell (CMA1))

Andere Bezeichnung: Chymase 1, Mast Cell (CMA1 Produkte)

Synonyme: CYH ELISA Kit, MCT1 ELISA Kit, chymase ELISA Kit, MMCP-5 ELISA Kit, Mcp-5 ELISA Kit, Mcp5 ELISA Kit, Mcpt5 ELISA Kit, MCP3P ELISA Kit, CMA1 ELISA Kit, Chymase ELISA Kit, chymase 1 ELISA Kit, chymase 1, mast cell ELISA Kit, chymase-like ELISA Kit, CMA1 ELISA Kit, Cma1 ELISA Kit, LOC100008644 ELISA Kit

Hintergrund:

Gene Name: Chymase 1, Mast Cell

Gene Aliases: CYH, CYM, Alpha-chymase, Mast cell protease I

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Carbohydrate Homeostasis