BID ELISA Kit
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- Target Alle BID ELISA Kits anzeigen
- BID (BH3 Interacting Domain Death Agonist (BID))
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Reaktivität
- Ratte
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 0.156 ng/mL - 10 ng/mL
- Untere Nachweisgrenze
- 0.156 ng/mL
- Applikation
- ELISA
- Proben
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytische Methode
- Quantitative
- Spezifität
- This assay has high sensitivity and excellent specificity for detection of BH3 Interacting Domain Death Agonist (Bid). No significant cross-reactivity or interference between BH3 Interacting Domain Death Agonist (Bid) and analogues was observed.
- Sensitivität
- 0.067 ng/mL
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- Kommentare
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Testdauer
- 3 h
- Plattentyp
- Pre-coated
- Protokoll
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to BH3 Interacting Domain Death Agonist (Bid). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to BH3 Interacting Domain Death Agonist (Bid). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain BH3 Interacting Domain Death Agonist (Bid), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of BH3 Interacting Domain Death Agonist (Bid) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Testpräzision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level BH3 Interacting Domain Death Agonist (Bid) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level BH3 Interacting Domain Death Agonist (Bid) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Handhabung
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Haltbarkeit
- 4-8 months
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- Target Alle BID ELISA Kits anzeigen
- BID (BH3 Interacting Domain Death Agonist (BID))
- Andere Bezeichnung
- BH3 Interacting Domain Death Agonist (BID Produkte)
- Synonyme
- FP497 ELISA Kit, 2700049M22Rik ELISA Kit, AI875481 ELISA Kit, AU022477 ELISA Kit, BID ELISA Kit, bid ELISA Kit, si:ch211-238n5.6 ELISA Kit, fp497 ELISA Kit, xbid ELISA Kit, DKFZp469E066 ELISA Kit, BH3 interacting domain death agonist ELISA Kit, BH3 interacting domain death agonist S homeolog ELISA Kit, BID ELISA Kit, Bid ELISA Kit, bida ELISA Kit, bid.S ELISA Kit, bid ELISA Kit
- Hintergrund
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Gene Name: BH3 Interacting Domain Death Agonist
Gene Aliases: p22 BID
- Gen-ID
- 64625
- UniProt
- Q9JLT6
- Pathways
- Apoptose, Caspase Kaskade in der Apoptose, Positive Regulation of Endopeptidase Activity
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