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ARNTL ELISA Kit

Dieses Human ARNTL ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human ARNTL zu quantifizieren.
Produktnummer ABIN5652028

Kurzübersicht für ARNTL ELISA Kit (ABIN5652028)

Target

Alle ARNTL ELISA Kits anzeigen
ARNTL (Aryl Hydrocarbon Receptor Nuclear Translocator-Like (ARNTL))

Reaktivität

  • 3
  • 1
  • 1
Human

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Detektionsbereich

0.312 ng/mL - 20 ng/mL

Applikation

ELISA

Proben

Cell Lysate, Tissue Homogenate
  • Untere Nachweisgrenze

    0.312 ng/mL

    Analytische Methode

    Quantitative

    Spezifität

    This assay has high sensitivity and excellent specificity for detection of Aryl Hydrocarbon Receptor Nuclear Translocator Like Protein (ARNTL). No significant cross-reactivity or interference between Aryl Hydrocarbon Receptor Nuclear Translocator Like Protein (ARNTL) and analogues was observed.

    Sensitivität

    0.114 ng/mL
  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer

    3 h

    Plattentyp

    Pre-coated

    Protokoll

    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aryl Hydrocarbon Receptor Nuclear Translocator Like Protein (ARNTL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aryl Hydrocarbon Receptor Nuclear Translocator Like Protein (ARNTL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aryl Hydrocarbon Receptor Nuclear Translocator Like Protein (ARNTL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aryl Hydrocarbon Receptor Nuclear Translocator Like Protein (ARNTL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

    Testpräzision

    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aryl Hydrocarbon Receptor Nuclear Translocator Like Protein (ARNTL) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aryl Hydrocarbon Receptor Nuclear Translocator Like Protein (ARNTL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Handhabung

    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

    Haltbarkeit

    4-8 months
  • Target Alle ARNTL ELISA Kits anzeigen

    ARNTL (Aryl Hydrocarbon Receptor Nuclear Translocator-Like (ARNTL))

    Andere Bezeichnung

    Aryl Hydrocarbon Receptor Nuclear Translocator Like Protein

    Hintergrund

    Gene Name: Aryl Hydrocarbon Receptor Nuclear Translocator Like Protein

    Gene Aliases: BMAL1, BMAL1c, JAP3, MOP3, PASD3, TIC, BHLHE5, Basic-helix-loop-helix-PAS protein MOP3, Brain and muscle ARNT-like 1, Class E basic helix-loop-helix protein 5

    Pathways

    Regulation of Lipid Metabolism by PPARalpha, Protein targeting to Nucleus, Warburg Effekt
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