APAF1 CLIA Kit

Produktnummer ABIN5651963

Maus APAF1 ELISA Kit, Chemiluminescent Assay für die Quantifizierung von Maus APAF1.

Kurzübersicht für APAF1 CLIA Kit (ABIN5651963)

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Reaktivität: Maus

Nachweismethode: Chemiluminescent

Methodentyp: Sandwich ELISA

Detektionsbereich: 13.7 pg/mL - 10000 pg/mL

Applikation: ELISA

Proben: Tissue Homogenate

Produktdetails APAF1 CLIA Kit

Untere Nachweisgrenze: 13.7 pg/mL

Verwendungszweck: Mouse Apoptotic Peptidase Activating Factor 1 (APAF1) CLIA Kit for detecting APAF1 in tissue homogenates

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Apoptotic Peptidase Activating Factor 1 (APAF1). No significant cross-reactivity or interference between Apoptotic Peptidase Activating Factor 1 (APAF1) and analogues was observed.

Sensitivität: 4.87 pg/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• Substrate A
• Substrate B
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The microplate provided in this kit has been pre-coated with an antibody specific to Apoptotic Peptidase Activating Factor 1 (APAF1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Apoptotic Peptidase Activating Factor 1 (APAF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Apoptotic Peptidase Activating Factor 1 (APAF1) level in the sample or standard.,

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apoptotic Peptidase Activating Factor 1 (APAF1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apoptotic Peptidase Activating Factor 1 (APAF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Andere Bezeichnung: Apoptotic Peptidase Activating Factor 1

Hintergrund: Gene Aliases: CED4, Apoptotic Protease Activating Factor

Gen-ID: 11783

UniProt: O88879

Pathways: p53 Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Tube Formation, Positive Regulation of Endopeptidase Activity