Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

ACVA ELISA Kit

ACVA Reaktivität: Maus Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Produktnummer ABIN5651389
  • Target Alle ACVA ELISA Kits anzeigen
    ACVA (Activin A (ACVA))
    Reaktivität
    • 5
    • 5
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Competition ELISA
    Detektionsbereich
    12.35 pg/mL - 1000 pg/mL
    Untere Nachweisgrenze
    12.35 pg/mL
    Applikation
    ELISA
    Proben
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Activin A (ACVA). No significant cross-reactivity or interference between Activin A (ACVA) and analogues was observed.
    Sensitivität
    4.97 pg/mL
    Featured
    Zu unserem meistverkauften ACVA ELISA Kit
    Top Product
    Discover our top product ACVA ELISA Kit
  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    2 h
    Plattentyp
    Pre-coated
    Protokoll
    This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Activin A (ACVA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Activin A (ACVA) and unlabeled Activin A (ACVA) (Standards or samples) with the pre-coated antibody specific to Activin A (ACVA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Activin A (ACVA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Activin A (ACVA) in the sample.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Activin A (ACVA) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Activin A (ACVA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle ACVA ELISA Kits anzeigen
    ACVA (Activin A (ACVA))
    Andere Bezeichnung
    Activin A (ACVA Produkte)
    Synonyme
    inhibin beta A subunit ELISA Kit, Inhba ELISA Kit
    Hintergrund

    Gene Name: Activin A

    Gene Aliases: ACV-A, Activin Beta A Beta A Homodimer

    Pathways
    Hormone Transport, Peptide Hormone Metabolism
Sie sind hier:
Kundenservice