HSD17B3 ELISA Kit (Hydroxysteroid (17-Beta) Dehydrogenase 3)

Details for Product HSD17B3 ELISA Kit No. ABIN5651262, Anbieter: Anmelden zum Anzeigen
Antigen
  • EDH17B3
  • SDR12C2
  • hydroxysteroid (17-beta) dehydrogenase 3
  • hydroxysteroid 17-beta dehydrogenase 3
  • Hsd17b3
  • HSD17B3
Reaktivität
Human
Alternativen
Kits mit alternativen Reaktivitäten:
5
3
2
Methodentyp
Sandwich ELISA
Detektionsbereich
15.62 pg/mL - 1000 pg/mL
Untere Nachweisgrenze
15.62 pg/mL
Applikation
ELISA
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
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Proben Plasma, Serum, Tissue Homogenate
Analytische Methode Quantitative
Nachweismethode Colorimetric
Spezifität This assay has high sensitivity and excellent specificity for detection of 17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17b3). No significant cross-reactivity or interference between 17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17b3) and analogues was observed.
Sensitivität 6.4 pg/mL
Plasmids, Primers & others Plasmide, Primers & weitere HSD17B3 products on genomics-online (e.g. as negative or positive controls)
Antigen
Andere Bezeichnung 17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17B3 ELISA Kit Abstract)
Hintergrund

Gene Name: 17-Beta-Hydroxysteroid Dehydrogenase Type 3

Gene Aliases: SDR12C2, EDH17B3, Short Chain Dehydrogenase/Reductase Family 12C,Member 2, Testosterone 17-Beta-Dehydrogenase 3, Testicular 17-beta-hydroxysteroid dehydrogenase

Gen-ID 3293
UniProt P37058
Pathways Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis
Kommentare

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer 3 h
Plattentyp Pre-coated
Protokoll The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17b3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17b3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17b3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17b3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Testpräzision Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17b3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17b3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Beschränkungen Nur für Forschungszwecke einsetzbar
Handhabung The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Haltbarkeit 4-8 months
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