HSD17B3 ELISA Kit

Produktnummer ABIN8090509

Human HSD17B3 ELISA Kit, Colorimetric Assay für die Quantifizierung von Human HSD17B3.

Kurzübersicht für HSD17B3 ELISA Kit (ABIN8090509)

Target: HSD17B3 (Hydroxysteroid (17-Beta) Dehydrogenase 3 (HSD17B3))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Detektionsbereich: 31.25 pg/mL - 2000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Produktdetails HSD17B3 ELISA Kit

Untere Nachweisgrenze: 31.25 pg/mL

Verwendungszweck: Human HSD17B3 (Testosterone 17-beta-dehydrogenase 3) ELISA Kit

Analytische Methode: Quantitative

Spezifität: Specifically binds with HSD17B3 , no obvious cross reaction with other analogues.

Sensitivität: 18.75 pg/mL

Bestandteile:

• ELISA Microplate(Dismountable)
• Lyophilized Standard
• Biotin-labeled Antibody
• HRP-Streptavidin Conjugate(SABC
• 100X)
• TMB Substrate
• Sample Dilution Buffer
• Stop Solution
• Plate Sealer
• Product Description

Benötigtes Material:

• Microplate reader (wavelength: 450nm)
• 37 °C incubator (CO2 incubator for cell culture is not recommenced.)
• Automated plate washer or multi-channel pipette/5 mL pipettor
• Precision single (0.5-10μL
• 5-50μL
• 20-200μL
• 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
• Sterile tubes and Eppendorf tubes with disposable tips
• Absorbent paper and loading slot
• Deionized or distilled water

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Competitive ELISA, Coated with Antigen

Probenmenge: 50 μL

Testdauer: 2 h

Plattentyp: Pre-coated

Protokoll: Step 1: Wash the plate twice before adding the standard and sample. Step 2: Add 50 µL standard or sample into each well. Immediately add 50 µL biotin-labeled antibody working solution into each well(The tip can't be reused after touching the liquid.), gently shake the plate for 1 min, seal closely and statically incubate for 45 minutes at 37 °C. Washing: Wash the plate three times and immerse for 1 min each time. Step 3: Add 100 µL HRP-Streptavidin Conjugate (SABC) working solution into each well, seal the plate and statically incubate for 30 minutes at 37 °C. Washing: Wash the plate five times and immerse for 1 min each time. Step 4: Add 90 µL TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37 °C. (Accurate TMB visualization control is required.) Step 5: Add 50 µL stop solution. Read at 450nm immediately and calculate.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid direct light exposure to antibody solutions

Lagerung: 4 °C

Informationen zur Lagerung: Store sealed Kit at 2-8° C. Do Not Cryopreserve. Store ELISA Microplate for 1 month at 2-8°C, Store for 12 month at -20°C. Put the leftover strips into a sealed foil bag with the desiccant

Haltbarkeit: 12 months

Details zu HSD17B3

Target: HSD17B3 (Hydroxysteroid (17-Beta) Dehydrogenase 3 (HSD17B3))

Andere Bezeichnung: HSD17B3

Hintergrund: 17-beta-hydroxysteroid dehydrogenase type 3, 17-beta-HSD 3, Estradiol 17-beta-dehydrogenase 2, Short chain dehydrogenase/reductase family 12C member 2, Testicular 17-beta-hydroxysteroid dehydrogenase, Testosterone 17-beta-dehydrogenase 3, HSD17B3, EDH17B3, SDR12C2

UniProt: P37058

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis