IL36A/IL1F6 ELISA Kit

Produktnummer ABIN5525085

Human IL36A/IL1F6 ELISA Kit, Colorimetric Assay für die Quantifizierung von Human IL36A/IL1F6.

Kurzübersicht für IL36A/IL1F6 ELISA Kit (ABIN5525085)

Target: IL36A/IL1F6 (IL1F6) (Interleukin 1 Family, Member 6 (IL1F6))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Produktdetails IL36A/IL1F6 ELISA Kit

Untere Nachweisgrenze: 0.156 ng/mL

Verwendungszweck: Human IL36A (Interleukin-36 alpha) ELISA Kit

Analytische Methode: Quantitative

Spezifität: Specifically binds with IL36A , no obvious cross reaction with other analogues. Recommended Sample Dilution Ratio The following table shows the recommended dilution ratios for this kit for a limited number of samples for your reference only. (The matrix components in serum/plasma will affect the test results, which it need to be diluted at least 1/2 with Sample Dilution Buffer before testing! When the content of other samples is very low, the original solution can be added without dilution, but it is necessary to ensure that the pH is between 6.8 and 8.0, and it does not contain more than 10 % organic solvents or high-concentration protein denaturants.) 1. Common sample validation: Sample Type Recommended Dilution Ratio Content Healthy serum (n=19) 1/2 dilution ND-0.22 ng/mL Healthy plasma (EDTA, Citrate , heparin) (n=20) 1/2 dilution ND-0.24 ng/mL Human peripheral blood mononuclear cells (2 x 10^6 cells/mL) were cultured in RPMI 1640 supplemented with 10 %FBS, 100 units/mL penicillin, and 100 μg/mL streptomycin sulfate for 48 hours. The cell culture supernatants were assayed for levels of human IL-36A. undiluted ND Human peripheral blood mononuclear cells (2 x 10^6 cells/mL) were cultured in RPMI 1640 supplemented with 10 %FBS, 100 units/mL penicillin, and 100 μg/mL streptomycin sulfate, followed by stimulated with 10 μg/mL PHA for 48 hours. The cell culture supernatants were assayed for levels of human IL-36A. undiluted 0.89 ng/mL 2. KD sample validation (Detect IL36A-KD PBMC cells): Sample Type Dilution Ratio Content Wild Human peripheral blood mononuclear cells (2 x 10^6 cells/mL) were cultured in RPMI 1640 supplemented with 10 %FBS, 100 units/mL penicillin, and 100 μg/mL streptomycin sulfate, followed by stimulated with 10 μg/mL PHA for 48 hours. The cell culture supernatants were assayed for levels of human IL-36A. undiluted 0.91 ng/mL Human peripheral blood mononuclear cells (+siRNA) were cultured in RPMI 1640 supplemented with 10 %FBS, 100 units/mL penicillin, and 100 μg/mL streptomycin sulfate, followed by stimulated with 10 μg/mL PHA for 48 hours. The cell culture supernatants were assayed for levels of human IL-36A. undiluted 0.12 ng/mL Note:ND is lower than the sensitivity of the kit and was not detected 3. Verified recombinant protein: (It is a normal phenomenon that some proteins have weak detection signals or cannot be detected at all due to differences in tags, sequences or protein activities) A. E.coli-derived Human IL-36A protein Lys6-Phe158

Sensitivität: 0.094 ng/mL

Bestandteile:

• ELISA Microplate(Dismountable)
• Lyophilized Standard
• Biotin-labeled Antibody
• HRP-Streptavidin Conjugate(SABC
• 100X)
• TMB Substrate
• Sample Dilution Buffer
• Stop Solution
• Plate Sealer
• Product Description

Benötigtes Material:

• Microplate reader (wavelength: 450nm)
• 37 °C incubator (CO2 incubator for cell culture is not recommenced.)
• Automated plate washer or multi-channel pipette/5 mL pipettor
• Precision single (0.5-10μL
• 5-50μL
• 20-200μL
• 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
• Sterile tubes and Eppendorf tubes with disposable tips
• Absorbent paper and loading slot
• Deionized or distilled water

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Sandwich ELISA, Double Antibody

Probenmenge: 100 μL

Testdauer: 4 h

Plattentyp: Pre-coated

Protokoll: Step 1: Add 100 µL standard or sample into each well, seal the plate and statically incubate for 90 minutes at 37 °C. Washing: Wash the plate twice without immersing. Step 2: Add 100 µL biotin-antibody working solution, seal the plate and statically incubate for 60 minutes at 37 °C. Washing: Wash the plate three times and immerse for 1 min each time. Step 3: Add 100 µL HRP-Streptavidin Conjugate (SABC) working solution, seal the plate and statically incubate for 30 minutes at 37 °C. Washing: Wash the plate five times and immerse for 1 min each time. Step 4: Add 90 µL TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37 °C. (Accurate TMB visualization control is required.) Step 5: Add 50 µL stop solution. Read at 450nm immediately and calculate.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid direct light exposure to antibody solutions

Lagerung: 4 °C

Informationen zur Lagerung: Store sealed Kit at 2-8° C. Do Not Cryopreserve. Store ELISA Microplate for 1 month at 2-8°C, Store for 12 month at -20°C. Put the leftover strips into a sealed foil bag with the desiccant

Haltbarkeit: 12 months

Details zu IL36A/IL1F6

Target: IL36A/IL1F6 (IL1F6) (Interleukin 1 Family, Member 6 (IL1F6))

Andere Bezeichnung: IL36A

Hintergrund: Interleukin-36 alpha, FIL1 epsilon, Interleukin-1 epsilon, IL-1 epsilon, Interleukin-1 family member 6, IL-1F6, IL36A, FIL1E, IL1E, IL1F6

UniProt: Q9UHA7

Pathways: Cancer Immune Checkpoints