FGF2 ELISA Kit

Produktnummer ABIN5068059

Human FGF2 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human FGF2.

Kurzübersicht für FGF2 ELISA Kit (ABIN5068059)

Target: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma (EDTA), Serum

Produktdetails FGF2 ELISA Kit

Verwendungszweck: This Human Fibroblast Growth Factor basic (FGF-b) ELISA kit is to be used for the quantitative determination of FGF basic in human serum, plasma, buffered solution, or cell culture medium. The assay will recognize both the natural 18kD isoform and recombinant FGF basic-146 and FGF basic-157.

Analytische Methode: Quantitative

Sensitivität: 7 pg/mL

Produktmerkmale: Fibroblast Growth Factor, Basic, Human (FGFb) ELISA Kit

Bestandteile:

• Microtiter Plate, 1x96wells. FGFb antibody-coated wells.
• Standard 2x1vial.
• Standard Diluent, 1x25ml. Contains 0.1% sodium azide.
• FGFb (Biotin) Pab, 1x11ml. Contains 0.1% sodium azide.
• Streptavidin (HRP), 100X, 1x125ul. Contains 3.3mM thymol.
• Streptavidin-HRP Diluent, 1x25ml. Contains 3.3mM thymol.
• Wash Buffer Concentrate, 25X, 1x100ml.
• TMB, 1x25ml.
• Stop Solution, 1x25ml.

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Principle of the Method:

• This human FGF basic kit is a solid phase sandwich ELISA Assay. A monoclonal antibody specific for human FGF basic has been coated onto the wells of the microtiter strips provided. Samples, including standards of known human FGF-b content, control specimens, and unknowns, are pipetted into these wells. During the first incubation, the FGF-b antigen binds to the immobilized (capture) antibody on one site. After washing, a biotinylated monoclonal antibody specific for FGF-b is added. During the second incubation, this antibody binds to the immobilized FGF-b captured during the first incubation. After removal of excess second antibody, Streptavidin-Peroxidase (enzyme) is added. This binds to the biotinylated antibody to complete the four-member sandwich. After a third incubation and washing to remove all the unbound enzyme, a substrate solution is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of FGF-b present in the original specimen.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 4°C

Details zu FGF2

Target: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Andere Bezeichnung: Fibroblast Growth Factor basic

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus, S100 Proteine