Albumin ELISA Kit

Produktnummer ABIN5067986

Dieses Maus Albumin ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Maus Albumin zu quantifizieren.

Kurzübersicht für Albumin ELISA Kit (ABIN5067986)

Target: Albumin (ALB)

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Proben: Cell Culture Cells, Saliva, Tissue Samples

Produktdetails Albumin ELISA Kit

Verwendungszweck: The Mouse Albumin ELISA Kit is an in vitro immunoassay for the quantification of albumin circulating in serum or in other appropriately qualified samples from tissue fluids (e.g., saliva, mucosa), or in cultures of mouse cells.

Analytische Methode: Quantitative

Kreuzreaktivität (Details): Calculated cross reactivity: Mo

Sensitivität: 10 ng/mL

Produktmerkmale: Albumin, Mouse, ELISA Kit

Bestandteile:

• Microtiter Plate: 1x96 wells
• Control (ng/ml), 1x650ul
• Standard (20ng/ml), 1x650ul
• Standard (50ng/ml), 1x650ul
• Standard (100ng/ml), 1x650ul
• Standard (200ng/ml), 1x650ul
• Standard (400ng/ml), 1x650ul
• HRP Conjugate (100X), 1x150ul
• Sample Diluent (20X), 1x10ml
• 0: Wash Solution (100X), 1x10ml
• 1: TMB Substrate, 1x12ml
• 2: Stop Solution, 1x12ml

Anwendungsinformationen

Protokoll: Principle:

• The Mouse Albumin ELISA kit is based on the binding of mouse albumin in samples to two antibodies, one immobilized on the microtiter wells, and the other conjugated to horseradish peroxidase (HRP) enzyme. After a washing step, chromogenic substrate is added and color is developed by the enzymatic reaction of HRP on the TMB substrate, which is directly proportional to the amount of albumin present in the sample. Stopping Solution is added to terminate the reaction, and absorbance at 450nm is then measured using an ELISA microtiter well reader. The concentration of albumin in samples and control is calculated from a curve of standards containing known concentrations of albumin.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 4°C

Details zu Albumin

Target: Albumin (ALB)

Andere Bezeichnung: Albumin

Pathways: Lipid Metabolism