beta Amyloid ELISA Kit

Produktnummer ABIN5067940

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human beta Amyloid.

Kurzübersicht für beta Amyloid ELISA Kit (ABIN5067940)

Target: beta Amyloid (Abeta) (Amyloid beta (Abeta))

Bindungsspezifität: AA 1-40

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Proben: Cerebrospinal Fluid, Tissue Homogenate, Tissue Culture Medium

Produktdetails beta Amyloid ELISA Kit

Verwendungszweck: The Human beta Amyloid 1-40 (Hu A beta40) ELISA kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). A monoclonal antibody specific for the NH2-terminus of Hu b-amyloid has been coated onto the wells of the microtiter strips provided. During the first incubation, standards of known Hu b-amyloid 40 content, controls, and unknown samples are pipetted into the wells and co-incubated with a rabbit antibody specific for the COOH-terminus of the 1-40 b-amyloid sequence. This COOH- terminal sequence is created upon cleavage of the analyzed precursor. Bound rabbit antibody is detected by the use of a horseradish peroxidase-labeled anti-rabbit antibody. After washing, horseradish peroxidase-labeled anti-rabbit antibody (enzyme) is added. After a second incubation and washing to remove all the unbound enzyme, a substrate solution is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of Hu A beta40 present in the original specimen.

Analytische Methode: Quantitative

Kreuzreaktivität (Details): Calculated cross reactivity: Hu

Sensitivität: > 6 pg/mL

Produktmerkmale: β-Amyloid, aa1-40, Human, ELISA Kit

Bestandteile:

• * beta Amyloid Standard, 1x1vial
• Standard Diluent Buffer, 1x60ml
• Microtiter Plate, 1x96 wells
• beta Amyloid , Pab 1x6ml
• IgG (HRP) (100X), 1x125ul
• HRP Diluent, 1x25ml
• Wash Buffer (25X), 1x100ml
• TMB, 1x25ml
• Stop Solution, 1x25ml

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: 4°C/-70°C

Details zu beta Amyloid

Target: beta Amyloid (Abeta) (Amyloid beta (Abeta))

Andere Bezeichnung: beta-Amyloid

Pathways: Inflammasome