PD-L1 ELISA Kit

Produktnummer ABIN5026969

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human PD-L1.

Kurzübersicht für PD-L1 ELISA Kit (ABIN5026969)

Target: PD-L1 (CD274 (PD-L1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156-10 ng/mL

Applikation: ELISA

Proben: Plasma, Serum

Produktdetails PD-L1 ELISA Kit

Untere Nachweisgrenze: 0.156 ng/mL

Verwendungszweck: This assay employs an antibody specific for Anti-human PD-L1 coated on a 96-well plate.

Analytische Methode: Quantitative

Sensitivität: 94 pg/mL

Produktmerkmale: This immunoassay kit allows for the in vitro quantitative determination of Human PD-L1 concentrations in serum, plasma. This kit is for Research use only. Not for use in diagnostic/therapeutics procedures. Detection Range: 0.156 - 10 ng/mL Sensitivity: < 94 pg/mL

Bestandteile: plate, standard, diluent, antibodies

Benötigtes Material: pipettes, tubes, reader, incubator

Anwendungsinformationen

Probenmenge: 100 μL

Testdauer: 3 - 4 h

Plattentyp: Pre-coated

Protokoll: This assay employs an antibody specific for Anti-human PD-L1 coated on a 96-well plate. Standards and samples are pipetted into the wells and PD-L1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human PD-L1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PD-L1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Vorsichtsmaßnahmen: protect your eyes

Lagerung: 4 °C

Informationen zur Lagerung: Please refer to the Manual

Details zu PD-L1

Target: PD-L1 (CD274 (PD-L1))

Andere Bezeichnung: PD-L1

Pathways: Cancer Immune Checkpoints