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PD-L1 ELISA Kit

PD-L1 Reaktivität: Maus Colorimetric Sandwich ELISA 0.156-10 ng/mL Plasma, Serum
Produktnummer ABIN5026968
  • Target Alle PD-L1 ELISA Kits anzeigen
    PD-L1 (CD274 (PD-L1))
    Reaktivität
    • 9
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.156-10 ng/mL
    Untere Nachweisgrenze
    0.156 ng/mL
    Applikation
    ELISA
    Verwendungszweck
    This immunoassay kit allows for the in vitro quantitative determination of Mouse PDL-1 concentrations in serum, plasma and other biological fluids.
    Proben
    Plasma, Serum
    Analytische Methode
    Quantitative
    Sensitivität
    < 0.094 ng/mL
    Bestandteile
    plate, standard, diluent, antibodies
    Benötigtes Material
    pipettes, tubes, reader, incubator
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  • Probenmenge
    100 μL
    Testdauer
    3 - 4 h
    Plattentyp
    Pre-coated
    Protokoll
    This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Mouse PDL-1 antibody was pre-coated into 96-well plates. Biotin conjugated anti-MousePDL-1detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse PDL-1 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450nm in a microplate reader. Concentration of Mouse PDL-1 can be calculated using the standard curve.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Vorsichtsmaßnahmen
    protect your eyes
    Lagerung
    4 °C
  • Target Alle PD-L1 ELISA Kits anzeigen
    PD-L1 (CD274 (PD-L1))
    Andere Bezeichnung
    PD-L1 (PD-L1 Produkte)
    Synonyme
    B7-H ELISA Kit, B7H1 ELISA Kit, PD-L1 ELISA Kit, PDCD1L1 ELISA Kit, PDCD1LG1 ELISA Kit, PDL1 ELISA Kit, A530045L16Rik ELISA Kit, B7h1 ELISA Kit, Pdcd1l1 ELISA Kit, Pdcd1lg1 ELISA Kit, Pdl1 ELISA Kit, RGD1566211 ELISA Kit, CD274 molecule ELISA Kit, CD274 antigen ELISA Kit, programmed cell death 1 ligand 2 ELISA Kit, CD274 ELISA Kit, Cd274 ELISA Kit, PDCD1LG2 ELISA Kit
    Pathways
    Cancer Immune Checkpoints
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