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IL-13 ELISA Kit

IL13 Reaktivität: Human Colorimetric Sandwich ELISA 15.6-1000 pg/mL Plasma, Serum
Produktnummer ABIN5026963
  • Target Alle IL-13 (IL13) ELISA Kits anzeigen
    IL-13 (IL13) (Interleukin 13 (IL13))
    Reaktivität
    • 12
    • 6
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    15.6-1000 pg/mL
    Untere Nachweisgrenze
    15.6 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    This immunoassay kit allows for the in vitro quantitative determination of Human IL-13 concentrations in serum, plasma and other biological fluids.
    Proben
    Plasma, Serum
    Analytische Methode
    Quantitative
    Sensitivität
    < 9.4 pg/mL
    Bestandteile
    plate, standard, diluent, antibodies
    Benötigtes Material
    pipettes, tubes, reader, incubator
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  • Probenmenge
    100 μL
    Testdauer
    3 - 4 h
    Plattentyp
    Pre-coated
    Protokoll
    This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human IL-13 antibody was pre-coated into 96-well plates. Biotin conjugated anti-human IL-13 detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IL-13 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450nm in a microplate reader. Concentration of Human IL-13 can be calculated using the standard curve.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Vorsichtsmaßnahmen
    protect your eyes
    Lagerung
    4 °C
  • Target Alle IL-13 (IL13) ELISA Kits anzeigen
    IL-13 (IL13) (Interleukin 13 (IL13))
    Andere Bezeichnung
    IL-13 (Interleukin 13) (IL13 Produkte)
    Synonyme
    IL13 ELISA Kit, IL-13 ELISA Kit, P600 ELISA Kit, Il-13 ELISA Kit, L13 ELISA Kit, interleukin 13 ELISA Kit, IL13 ELISA Kit, Il13 ELISA Kit
    Pathways
    JAK-STAT Signalweg, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport
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