PD-1 ELISA Kit

Produktnummer ABIN5026948

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human PD-1.

Kurzübersicht für PD-1 ELISA Kit (ABIN5026948)

Target: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156-10 ng/mL

Applikation: ELISA

Proben: Plasma, Serum

Produktdetails PD-1 ELISA Kit

Untere Nachweisgrenze: 0.156 ng/mL

Verwendungszweck: This kit was based on sandwich enzyme-linked immune-sorbent assay technology.

Analytische Methode: Quantitative

Sensitivität: 0.094 ng/mL

Produktmerkmale: This immunoassay kit allows for the in vitro quantitative determination of Human PD-1 concentrations in serum, plasma. Detection Range: 0.156 - 10 ng/mL Sensitivity: < 0.094 ng/mL

Bestandteile: plate, standard, diluent, antibodies

Benötigtes Material: pipettes, tubes, reader, incubator

Anwendungsinformationen

Probenmenge: 100 μL

Testdauer: 3 - 4 h

Plattentyp: Pre-coated

Protokoll: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human PD-1 antibody was pre-coated into 96-well plates. Biotin conjugated anti-human PD-1 detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human PD-1 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450 nm in a microplate reader. Concentration of Human PD-1 can be calculated using the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Vorsichtsmaßnahmen: protect your eyes

Lagerung: 4 °C

Informationen zur Lagerung: Please refer to the Manual

Details zu PD-1

Target: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Andere Bezeichnung: PD-1 (Programmed Cell Death Protein1)

Pathways: Cancer Immune Checkpoints