IL-5 ELISA Kit

Produktnummer ABIN5026941

Human IL-5 ELISA Kit, Colorimetric Assay für die Quantifizierung von Human IL-5.

Kurzübersicht für IL-5 ELISA Kit (ABIN5026941)

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.6-1000 pg/mL

Applikation: ELISA

Proben: Plasma, Serum

Produktdetails IL-5 ELISA Kit

Untere Nachweisgrenze: 15.6 pg/mL

Verwendungszweck: This assay employs an antibody specific for Anti-human IL-5 coated on a 96-well plate.

Analytische Methode: Quantitative

Sensitivität: 9.4 pg/mL

Produktmerkmale: This immunoassay kit allows for the in vitro quantitative determination of Human IL-5 concentrations in serum, plasma. Detection Range: 15.6 - 1000 pg/mL Sensitivity: < 9.4 pg/mL

Bestandteile: plate, standard, diluent, antibodies

Benötigtes Material: pipettes, tubes, reader, incubator

Anwendungsinformationen

Probenmenge: 100 μL

Testdauer: 3 - 4 h

Plattentyp: Pre-coated

Protokoll: This assay employs an antibody specific for Anti-human IL-5 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL-5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. 

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Vorsichtsmaßnahmen: protect your eyes

Lagerung: 4 °C

Informationen zur Lagerung: Please refer to the Manual

Details zu IL-5

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Andere Bezeichnung: IL-5 (Interleukin 5)

Pathways: JAK-STAT Signalweg, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour