MMP2 ELISA Kit
Kurzübersicht für MMP2 ELISA Kit (ABIN5026923)
Target
Alle MMP2 ELISA Kits anzeigenReaktivität
Nachweismethode
Methodentyp
Detektionsbereich
Applikation
Proben
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Untere Nachweisgrenze
- 0.781 ng/mL
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Verwendungszweck
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology.
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Analytische Methode
- Quantitative
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Sensitivität
- 0.469 ng/mL
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Produktmerkmale
- This immunoassay kit allows for the in vitro quantitative determination of Human MMP-2 concentrations in serum, plasma. Detection Range: 0.781 - 50 ng/mL Sensitivity: < 0.469 ng/mL
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Bestandteile
- plate, standard, diluent, antibodies
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Benötigtes Material
- pipettes, tubes, reader, incubator
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Matrix Metalloproteinase 2 (MMP2) ELISA Kit
MMP2 Reaktivität: Human Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 Reaktivität: Ratte Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 Reaktivität: Human Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 Reaktivität: Maus Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 Reaktivität: Pferd Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 Reaktivität: Schwein Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 Reaktivität: Maus Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 Reaktivität: Human Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 Reaktivität: Schwein Colorimetric Sandwich ELISA 47 ng/mL - 3000 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 Reaktivität: Human Colorimetric Sandwich ELISA 0.5 ng/mL - 32 ng/mL Plasma, Serum, Tissue Homogenate
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Probenmenge
- 100 μL
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Testdauer
- 3 - 4 h
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Plattentyp
- Pre-coated
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Protokoll
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human MMP-2 antibody was pre-coated into 96-well plates. Biotin conjugated anti-human MMP-2 detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human MMP-2 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450nm in a microplate reader. Concentration of Human MMP-2 can be calculated using the standard curve.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Vorsichtsmaßnahmen
- protect your eyes
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Lagerung
- 4 °C
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Informationen zur Lagerung
- Please refer to the Manual
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- MMP2 (Matrix Metalloproteinase 2 (MMP2))
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Andere Bezeichnung
- MMP-2 (Matrix Metalloproteinase 2)
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Pathways
- Activation of Innate immune Response
Target Alle MMP2 ELISA Kits anzeigen
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