IL-5 ELISA Kit

Produktnummer ABIN4986959

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human IL-5.

Kurzübersicht für IL-5 ELISA Kit (ABIN4986959)

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 3.9-250 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate), Plasma (EDTA)

Produktdetails IL-5 ELISA Kit

Untere Nachweisgrenze: 3.9 pg/mL

Analytische Methode: Quantitative

Spezifität: Natural and recombinant Human IL-5 Ligand

Sensitivität: 2 pg/mL

Benötigtes Material:

• Microplate reader.
• Pipettes and pipette tips.
• EP tube Deionized or distilled water.

Anwendungsinformationen

Applikationshinweise: Detection Wavelength: 450 nm

Probenmenge: 20 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Details zu IL-5

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Andere Bezeichnung: IL-5

Hintergrund: Interleukin-5(IL-5)is a secreted glycoprotein that belongs to the α-helical group of cytokines (1-3). Unlike other family members, it is present as a covalently linked antiparallel dimer (4, 5). The cDNA for human IL-5 encodes a signal peptide and a 115 amino acid (aa) mature protein. Mature human IL-5 shares 70 % , 70 %, 62 %, 71 %, 70 % and 66 %, aa sequence identity with mouse, rat, canine, equine, feline and porcine IL-5, respectively and shows cross-reactivity with mouse IL-5. IL-5 is primarily produced by CD4+ Th2 cells, but also by activated eosinophils, mast cells, EBV-transformed B cells, Reed-Sternberg cells in Hodgkin

Pathways: JAK-STAT Signalweg, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour