IL-15 ELISA Kit

Produktnummer ABIN4986933

Dieses Human IL-15 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human IL-15 zu quantifizieren.

Kurzübersicht für IL-15 ELISA Kit (ABIN4986933)

Target: IL-15 (IL15) (Interleukin 15 (IL15))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 7.8-500 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate), Plasma (EDTA)

Produktdetails IL-15 ELISA Kit

Untere Nachweisgrenze: 7.8 pg/mL

Analytische Methode: Quantitative

Spezifität: Natural and recombinant Human IL-15 Ligand

Sensitivität: 4 pg/mL

Benötigtes Material:

• Microplate reader.
• Pipettes and pipette tips.
• EP tube Deionized or distilled water.

Anwendungsinformationen

Applikationshinweise: Detection Wavelength: 450 nm

Probenmenge: 20 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Details zu IL-15

Target: IL-15 (IL15) (Interleukin 15 (IL15))

Andere Bezeichnung: IL-15

Hintergrund: Interleukin 15 (IL-15) is a 14 kDa cytokine that is structurally and functionally related to IL- 2(1-3). Mature human IL-15 shares 70 % amino acid sequence identity with mouse and rat IL-15. Alternate splicing generates isoforms of IL-15 with either a long or short signal peptide (LSP or SSP), and the SSP isoform is retained intracellularly (4). IL-15 associates with IL-15 Rα in the endoplasmic reticulum, and this complex is expressed on the cell surface (5, 6). The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15 Rα are coordinately expressed on the surface of one cell and interact with complexes of IL-2 Rβ/γc on adjacent cells (7). This enables cells to respond to IL-15 even if they do not express IL-15 Rα (6, 8). Consistent with its shared use of IL-2 receptor subunits, IL-15 induces IL-2-like effects in lymphocyte development and homeostasis (3). It is particularly important for the maintenance and activation of NK cells and CD8+ memory T cells (3). IL-15 also exerts pleiotropic effects on other hematopoietic cells and nonimmune cells (2). Ligation of membraneassociated IL-15/IL-15Rα complexes induces reverse signaling that promotes cellular adhesion, tyrosine phosphorylation of intracellular proteins, and cytokine secretion by the IL-15/IL-15Rα expressing cells (9, 10).

Pathways: JAK-STAT Signalweg, Glycosaminoglycan Metabolic Process