Interferon gamma ELISA Kit

Produktnummer ABIN4986921

Dieses Schwein Interferon gamma ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Schwein Interferon gamma zu quantifizieren.

Kurzübersicht für Interferon gamma ELISA Kit (ABIN4986921)

Target: Interferon gamma (IFNG)

Reaktivität: Schwein

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.02-1.0 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate), Plasma (EDTA)

Produktdetails Interferon gamma ELISA Kit

Untere Nachweisgrenze: 0.02 ng/mL

Analytische Methode: Quantitative

Spezifität: Natural and recombinant Swine IFN-γ Ligand

Sensitivität: 7 pg/mL

Benötigtes Material:

• Microplate reader.
• Pipettes and pipette tips.
• EP tube Deionized or distilled water.

Anwendungsinformationen

Applikationshinweise: Detection Wavelength: 450 nm

Probenmenge: 20 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Details zu Interferon gamma

Target: Interferon gamma (IFNG)

Andere Bezeichnung: IFN-gamma

Hintergrund: Interferon-gamma (IFN- , also known as type II interferon) is an important immunoregulatory cytokine that was originally identified because of its anti-viral activity (1). It plays key roles in host defense by exerting antiviral, antiproliferative and immunoregulatory activities (2 - 5). On many cell types, IFN- induces the production of cytokines and upregulates the expression of various membrane proteins including class I and II MHC antigens, Fc receptors, leukocyte adhesion molecules and B7 family antigens. IFN- is a potent activator of macrophage effector functions. It potentiates the secretion of immunoglobulins by B cells, and directs the synthesis of IgG. IFN- also influences T-helper cell phenotype development by inhibiting Th2 differentiation and stimulating Th1 development (2 - 5). Finally, IFN- promotes mononuclear cell chemotaxis by inducing the synthesis of CXCL9, CXCL10, CCL2, CCL3, CCL4, and CCL5(3). IFN- is produced by a number of cell types, including dendritic epidermal/ T cells (6), keratinocytes (7), peripheral blood T cells (8), mast cells (9), neurons (10), CD8 T cells (11), macrophages (12), B cells (13), neutrophils (14), NK cells (15), CD4 T cells (16) and testicular spermatids (17). The production of IFN- is upregulated synergistically by IL-12, IL-18, IL-23 and IL-27 (18 - 21). Porcine IFN- cDNA encodes a 166 amino acid (aa) residue precursor protein with a 20 aa signal sequence that is cleaved to generate a 146 aa residue mature IFN- (22, 23). Porcine IFN- is presumably a noncovalently linked homodimer (3). In the mature segment, porcine IFN- shares 60 % , 55 %, 41 %, 42 %, 72 %, and 72 % aa sequence identity with human (24), guinea pig (25), mouse (26), rat (27), feline (28), and canine (29) IFN- , respectively.The functional IFN- receptor complex consists of two distinct subunits (30). The alpha-subunit(IFN- R1) binds IFN- with high affinity and species specificity. The beta-subunit [IFN- R2, also known as accessory factor-1 (AF-1)] interacts with the IFN- occupied-subunit in a species-specific manner and participates in JAK-STAT mediated signal transduction. Although the functional receptor is suggested to consist of homodimeric IFN- in combination with two-chains, and two-chains (30, 31), it has been suggested that additional subunits may be involved (32, 33). Whereas the-chain is expressed constitutively on many cell types, the cellular regulation of the-chain correlates with an IFN- responsive state and is tightly regulated (30).

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagie