Gastrin-Releasing Peptide ELISA Kit

Produktnummer ABIN4889773

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human Gastrin-Releasing Peptide.

Kurzübersicht für Gastrin-Releasing Peptide ELISA Kit (ABIN4889773)

Target: Gastrin-Releasing Peptide (GRP)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Detektionsbereich: 0.1 ng/mL - 1000 ng/mL

Applikation: ELISA

Proben: Plasma, Serum

Produktdetails Gastrin-Releasing Peptide ELISA Kit

Untere Nachweisgrenze: 0.1 ng/mL

Verwendungszweck: Human GRP EIA Kit optimized for serum, plasma, and cell culture supernatants. Competition-based ELISA

Analytische Methode: Quantitative

Spezifität: This EIA kit is designed to detect human Gastrin-releasing peptide.

Sensitivität: 0.4 ng/mL

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Standard Peptide
• Assay Diluent(s)
• Biotinylated Peptide
• HRP-Streptavidin
• TMB One-Step Substrate
• Stop Solution
• Assay Diagram
• Positive Control Sample
• Capture Antibody
• User Manual

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Standard Peptide
• Assay Diluent(s)
• Biotinylated Peptide
• HRP-Streptavidin
• TMB One-Step Substrate
• Stop Solution
• Assay Diagram
• Positive Control Sample
• Capture Antibody
• User Manual

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Orbital shaker
• Aluminum foil
• Saran Wrap
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• SigmaPlot software (or other software that can perform four-parameter logistic regression models)

Anwendungsinformationen

Applikationshinweise: Suggested Plasma Dilution: Human: 2X

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL detection antibody to each well.
3. Incubate 1.5 h at RT or O/N at 4 °C.
4. Add 100 μL standard or sample to each well.
5. Incubate 2.5 h at RT.
6. Add 100 μL prepared streptavidin solution.
7. Incubate 45 min at RT.
8. Add 100 μL TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL Stop Solution to each well.
11. Read plate at 450 nm immediately.

Aufbereitung der Reagenzien:

Recommended Dilution for serum and plasma samplesHuman: 2x / Mouse: 8x / Rat: 2x

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.

Haltbarkeit: 6 months

Details zu Gastrin-Releasing Peptide

Target: Gastrin-Releasing Peptide (GRP)

Andere Bezeichnung: GRP

Hintergrund:

Gene Symbols: GRP

Background: Gastrin-releasing peptide (GRP), is a 27-amino acid neuropeptide. Gastrin-releasing peptide stimulates the release of Gastrin from the G cells of the stomach, and regulates gastric acid secretion and enteric motor function. Gastrin-releasing peptide is also an important growth-modulating factor in developing lung epithelium. It has been used as a tumor marker in the diagnosis of small-cell lung carcinoma, since it is known to be produced by these cancer cells. GRP is also involved in the biology of the circadian system, playing a role in the signaling of light to the master circadian oscillator in the suprachiasmatic nuclei of the hypothalamus. Furthermore, GRP seems to mediate certain aspects of stress.

Protein Name (Synonyms): Gastrin-releasing peptide (GRP) [Cleaved into: Neuromedin-C (GRP-10)]

Gen-ID: 2922

UniProt: P07492

Pathways: Peptide Hormone Metabolism, Hormone Activity