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PDPK1 ELISA Kit

PDPK1 Reaktivität: Maus, Human, Ratte pSer241 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Produktnummer ABIN4886246
  • Target Alle PDPK1 ELISA Kits anzeigen
    PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))
    Bindungsspezifität
    pSer241, total
    Reaktivität
    Maus, Human, Ratte
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human, Mouse and Rat Phospho-PDK1 (Ser241) and Total PDK1 ELISA Kit. This assay semi-quantitatively measures phophorylated PDK1 (Ser241) and Total PDK1 in lysate samples.
    Proben
    Cell Lysate, Tissue Lysate
    Analytische Methode
    Semi-Quantitative
    Spezifität
    This ELISA kit recognizes Human, Mouse and Rat PDK1 phosphorylated at site Serine-241 as well as total PDK1.
    Produktmerkmale
    • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • Anti-Pan Antibody
    • HRP-Conjugated Secondary Antibody
    • Streptavidin-Conjugated HRP
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Benötigtes Material
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
  • Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Haltbarkeit
    6 months
  • Target Alle PDPK1 ELISA Kits anzeigen
    PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))
    Andere Bezeichnung
    PDK1 (PDPK1 Produkte)
    Synonyme
    pdpk1 ELISA Kit, MGC82080 ELISA Kit, PDK1 ELISA Kit, PDPK2 ELISA Kit, PRO0461 ELISA Kit, Pdk1 ELISA Kit, zgc:153787 ELISA Kit, 3'-phosphoinositide-dependent protein kinase 1 ELISA Kit, 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1 ELISA Kit, ATPDK1 ELISA Kit, AtPDK1 ELISA Kit, T32M21.110 ELISA Kit, T32M21_110 ELISA Kit, zgc:77318 ELISA Kit, 3-phosphoinositide dependent protein kinase 1 ELISA Kit, 3-phosphoinositide dependent protein kinase 1 L homeolog ELISA Kit, 3-phosphoinositide dependent protein kinase-1 ELISA Kit, 3-phosphoinositide dependent protein kinase 1a ELISA Kit, 3-phosphoinositide-dependent protein kinase 1 ELISA Kit, 3'-phosphoinositide-dependent protein kinase 1 ELISA Kit, 3-phosphoinositide dependent protein kinase 1b ELISA Kit, PDPK1 ELISA Kit, pdpk1.L ELISA Kit, pdpk1 ELISA Kit, Pdpk1 ELISA Kit, pdpk1a ELISA Kit, LOC100380750 ELISA Kit, pdk-1 ELISA Kit, PDK1 ELISA Kit, pdpk1b ELISA Kit
    Gen-ID
    5170
    UniProt
    O15530, Q9Z2A0, O55173
    Pathways
    PI3K-Akt Signalweg, T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals
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