FAK ELISA Kit

Produktnummer ABIN4886093

Produktdetails FAK ELISA Kit

Target: FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))

Bindungsspezifität: pTyr

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Custom Human Phosphotyrosine FAK ELISA Kit. This assay semi-quantitatively measures phosphotyrosine FAK in lysate samples.

Proben: Cell Lysate, Tissue Lysate

Analytische Methode: Semi-Quantitative

Spezifität: The antibody pair provided in this kit recognizes Human Tyrosine-Phosphorylated-FAK.

Produktmerkmale:

• Rapidly measure phosphorylated protein in lysates
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Biotinylated Anti-Phosphotyrosine Antibody
• Stop Solution
• Assay Diluent(s)
• Positive Control Sample
• Lysis Buffer
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Haltbarkeit: 6 months

Details zu FAK

Target: FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))

Andere Bezeichnung: FAK (PTK2 Produkte)

Synonyme: FADK ELISA Kit, FAK ELISA Kit, FAK1 ELISA Kit, FRNK ELISA Kit, PPP1R71 ELISA Kit, p125FAK ELISA Kit, pp125FAK ELISA Kit, PTK2 ELISA Kit, Fadk ELISA Kit, mKIAA4203 ELISA Kit, fak ELISA Kit, fak1a ELISA Kit, ptk2 ELISA Kit, ptk2a ELISA Kit, wu:fa18c04 ELISA Kit, zgc:66114 ELISA Kit, CG10023 ELISA Kit, CT28129 ELISA Kit, DFAK ELISA Kit, DFak ELISA Kit, DFak56 ELISA Kit, Dfak ELISA Kit, Dfak56 ELISA Kit, DmFAK ELISA Kit, Dmel\\CG10023 ELISA Kit, Fak56 ELISA Kit, Fak56D ELISA Kit, fak56 ELISA Kit, protein tyrosine kinase 2 ELISA Kit, protein tyrosine kinase 2 L homeolog ELISA Kit, PTK2 protein tyrosine kinase 2 ELISA Kit, protein tyrosine kinase 2ab ELISA Kit, Focal adhesion kinase ELISA Kit, PTK2 ELISA Kit, ptk2.L ELISA Kit, Ptk2 ELISA Kit, ptk2ab ELISA Kit, Fak ELISA Kit

Gen-ID: 5747

UniProt: Q05397

Pathways: Response to Growth Hormone Stimulus, CXCR4-mediated Signaling Events, Smooth Muscle Cell Migration, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling