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ERBB4 ELISA Kit

ERBB4 Reaktivität: Human pTyr Colorimetric Sandwich ELISA Cell Culture Lysate
Produktnummer ABIN4886087
  • Target Alle ERBB4 ELISA Kits anzeigen
    ERBB4 (V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4))
    Bindungsspezifität
    pTyr
    Reaktivität
    • 4
    • 2
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human Phosphotyrosine ErbB4 ELISA Kit. This assay semi-quantitatively measures phosphotyrosine ErbB4 in lysate samples.
    Proben
    Cell Culture Lysate
    Analytische Methode
    Semi-Quantitative
    Spezifität
    This ELISA kit recognizes human ErbB4 phosphotyrosine.
    Produktmerkmale
    • Rapidly measure phosphorylated protein in lysates
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Biotinylated Anti-Phosphotyrosine Antibody
    • Stop Solution
    • Assay Diluent(s)
    • Positive Control Sample
    • Lysis Buffer
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
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  • Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Haltbarkeit
    6 months
  • Target Alle ERBB4 ELISA Kits anzeigen
    ERBB4 (V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4))
    Andere Bezeichnung
    ErbB4 (ERBB4 Produkte)
    Synonyme
    erbb4 ELISA Kit, si:ch211-227n20.1 ELISA Kit, Her4 ELISA Kit, c-erbB-4 ELISA Kit, HER4 ELISA Kit, p180erbB4 ELISA Kit, erb-b2 receptor tyrosine kinase 4 ELISA Kit, erb-b2 receptor tyrosine kinase 4a ELISA Kit, v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian) ELISA Kit, erb-b2 receptor tyrosine kinase 4 L homeolog ELISA Kit, receptor tyrosine-protein kinase ErbB-4 ELISA Kit, ERBB4 ELISA Kit, erbb4a ELISA Kit, erbb4 ELISA Kit, erbb4.L ELISA Kit, Tsp_13582 ELISA Kit, Erbb4 ELISA Kit
    Gen-ID
    2066
    UniProt
    Q15303
    Pathways
    RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung
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