EPH Receptor A2 ELISA Kit

Produktnummer ABIN4886062

Produktdetails EPH Receptor A2 ELISA Kit

Target: EPH Receptor A2 (EPHA2)

Bindungsspezifität: pTyr

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Custom Human Phosphotyrosine EphA2 ELISA Kit. This assay semi-quantitatively measures phosphotyrosine EphA2 in lysate samples.

Proben: Cell Lysate, Tissue Lysate

Analytische Methode: Semi-Quantitative

Spezifität: The antibody pair provided in this kit recognizes Human Tyrosine-Phosphorylated-EphA2.

Produktmerkmale:

• Rapidly measure phosphorylated protein in lysates
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Biotinylated Anti-Phosphotyrosine Antibody
• Stop Solution
• Assay Diluent(s)
• Positive Control Sample
• Lysis Buffer
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Haltbarkeit: 6 months

Details zu EPH Receptor A2

Target: EPH Receptor A2 (EPHA2)

Andere Bezeichnung: EphA2 (EPHA2 Produkte)

Synonyme: ARCC2 ELISA Kit, CTPA ELISA Kit, CTPP1 ELISA Kit, CTRCT6 ELISA Kit, ECK ELISA Kit, epha2 ELISA Kit, MGC89505 ELISA Kit, AW545284 ELISA Kit, Eck ELISA Kit, Myk2 ELISA Kit, Sek-2 ELISA Kit, Sek2 ELISA Kit, id:ibd5152 ELISA Kit, rtk6 ELISA Kit, G42 ELISA Kit, XE10 ELISA Kit, epha2-A ELISA Kit, EPH receptor A2 ELISA Kit, Eph receptor A2 ELISA Kit, epoxide hydrolase ELISA Kit, eph receptor A2 a ELISA Kit, EPH receptor A2 L homeolog ELISA Kit, EPHA2 ELISA Kit, Epha2 ELISA Kit, epha2 ELISA Kit, ephA2 ELISA Kit, epha2a ELISA Kit, epha2.L ELISA Kit

Gen-ID: 3386

UniProt: P29317

Pathways: RTK Signalweg