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TPP1 ELISA Kit

TPP1 Reaktivität: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN4884857
  • Target Alle TPP1 ELISA Kits anzeigen
    TPP1 (Tripeptidyl Peptidase I (TPP1))
    Reaktivität
    • 3
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Custom Human Tripeptidyl-peptidase I (TPP1) ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Spezifität
    This ELISA antibody pair recognizes human Tripeptidyl-peptidase I. Other species not yet determined.
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle TPP1 ELISA Kits anzeigen
    TPP1 (Tripeptidyl Peptidase I (TPP1))
    Andere Bezeichnung
    Tripeptidyl-Peptidase I (TPP1 Produkte)
    Synonyme
    cln2 ELISA Kit, fa01b09 ELISA Kit, im:7149243 ELISA Kit, wu:fa01b09 ELISA Kit, CLN2 ELISA Kit, LPIC ELISA Kit, TPP-1 ELISA Kit, TPP-I ELISA Kit, Cln2 ELISA Kit, tripeptidyl peptidase I ELISA Kit, tripeptidyl-peptidase 1 ELISA Kit, tripeptidyl peptidase 1 ELISA Kit, tpp1 ELISA Kit, NAEGRDRAFT_78259 ELISA Kit, MCYG_00184 ELISA Kit, MGYG_05881 ELISA Kit, MGYG_00757 ELISA Kit, TPP1 ELISA Kit, Tpp1 ELISA Kit
    Gen-ID
    1200
    UniProt
    O14773
    Pathways
    Zellzyklus, ER-Nucleus Signaling
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