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IL1RAP ELISA Kit

IL1RAP Reaktivität: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN4883354
  • Target Alle IL1RAP ELISA Kits anzeigen
    IL1RAP (Interleukin 1 Receptor Accessory Protein (IL1RAP))
    Reaktivität
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Custom Human IL-1R3 ELISA Kit..
    Proben
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Featured
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  • Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle IL1RAP ELISA Kits anzeigen
    IL1RAP (Interleukin 1 Receptor Accessory Protein (IL1RAP))
    Andere Bezeichnung
    IL-1 R3 (IL1RAP Produkte)
    Synonyme
    IL1RAP ELISA Kit, il-1racp ELISA Kit, il-racp ELISA Kit, il1r3 ELISA Kit, C3orf13 ELISA Kit, IL-1RAcP ELISA Kit, IL1R3 ELISA Kit, 6430709H04Rik ELISA Kit, AI255955 ELISA Kit, AV239853 ELISA Kit, IL-1RAcp ELISA Kit, Il1racpb ELISA Kit, interleukin 1 receptor accessory protein ELISA Kit, IL1RAP ELISA Kit, il1rap ELISA Kit, il-1racp ELISA Kit, Il1rap ELISA Kit
    Gen-ID
    3556
    UniProt
    Q9NPH3
    Pathways
    NF-kappaB Signalweg, Growth Factor Binding
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