GCK ELISA Kit

Produktnummer ABIN4883035

Produktdetails GCK ELISA Kit

Target: GCK (Glucokinase (Hexokinase 4) (GCK))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Custom Human Glucokinase/GCK ELISA Kit.

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes .

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu GCK

Target: GCK (Glucokinase (Hexokinase 4) (GCK))

Andere Bezeichnung: Glucokinase (GCK Produkte)

Synonyme: FGQTL3 ELISA Kit, GK ELISA Kit, GLK ELISA Kit, HHF3 ELISA Kit, HK4 ELISA Kit, HKIV ELISA Kit, HXKP ELISA Kit, LGLK ELISA Kit, MODY2 ELISA Kit, Gk ELISA Kit, Gls006 ELISA Kit, GCK ELISA Kit, BA4487 ELISA Kit, glk ELISA Kit, hk4 ELISA Kit, hhf3 ELISA Kit, hkiv ELISA Kit, hxkp ELISA Kit, gck-A ELISA Kit, mody2 ELISA Kit, glucokinase ELISA Kit, GLUKA ELISA Kit, RNGK2 ELISA Kit, glucokinase ELISA Kit, glucokinase (hexokinase 4) L homeolog ELISA Kit, glucokinase (hexokinase 4) ELISA Kit, GCK ELISA Kit, Gck ELISA Kit, glcK ELISA Kit, Bphyt_1025 ELISA Kit, Halhy_6548 ELISA Kit, gk ELISA Kit, gck.L ELISA Kit, gck ELISA Kit

Gen-ID: 2645

UniProt: P35557

Pathways: MAPK Signalweg, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process