COMT ELISA Kit

Produktnummer ABIN4882446

Produktdetails COMT ELISA Kit

Target: COMT (Catechol-O-Methyltransferase (COMT))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Custom Human COMT ELISA Kit.

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Human LTA4H.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu COMT

Target: COMT (Catechol-O-Methyltransferase (COMT))

Andere Bezeichnung: COMT (COMT Produkte)

Synonyme: Comt1 ELISA Kit, D16Wsu103e ELISA Kit, D330014B15Rik ELISA Kit, COMT ELISA Kit, mb-comt ELISA Kit, s-comt ELISA Kit, comt ELISA Kit, MGC154444 ELISA Kit, zgc:114157 ELISA Kit, si:dkey-13a21.15 ELISA Kit, zK13A21.15 ELISA Kit, zgc:162236 ELISA Kit, catechol-O-methyltransferase ELISA Kit, microRNA 4761 ELISA Kit, catechol-O-methyltransferase S homeolog ELISA Kit, methyltransferase ELISA Kit, catechol-o-methyltransferase ELISA Kit, catechol-O-methyltransferase a ELISA Kit, catechol-O-methyltransferase b ELISA Kit, catechol O-methyltransferase ELISA Kit, COMT ELISA Kit, Comt ELISA Kit, MIR4761 ELISA Kit, comt ELISA Kit, comt.2 ELISA Kit, comt.S ELISA Kit, Rv1703c ELISA Kit, Mb1729c ELISA Kit, comta ELISA Kit, comtb ELISA Kit, LOC100354142 ELISA Kit

Gen-ID: 1312

UniProt: P21964

Pathways: Steroid Hormone Biosynthesis, SARS-CoV-2 Protein Interaktom