COMT ELISA Kit

Produktnummer ABIN6720158

Produktdetails COMT ELISA Kit

Target: COMT (Catechol-O-Methyltransferase (COMT))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.2 pg/mL - 2000 pg/mL

Untere Nachweisgrenze: 31.2 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich ELISA for Quantitative Detection of Antigen

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytische Methode: Quantitative

Produktmerkmale:

Synonyms: Catechol O methyltransferase, Catechol O-methyltransferase, COMT, COMT_HUMAN, EC 2.1.1.6

Background: Catechol-O-methyltransferase (COMT) is one of several enzymes that degrade catecholamines such as dopamine, epinephrine, and norepinephrine. In humans, catechol-O-methyltransferase protein is encoded by the COMT gene. As the regulation of catecholamines is impaired in a number of medical conditions, several pharmaceutical drugs target COMT to alter its activity and therefore the availability of catecholamines. In the brain, COMT-dependent dopamine degradation is of particular importance in brain regions with low expression of the presynaptic dopamine transporter (DAT), such as the prefrontal cortex. This process is supposed to take place in postsynaptic neurons, as, in general, COMT is located intracellularly in the central nervous system (CNS).

Gene Name: COMT

Production: Natural and recombinant human COMT. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: G52-P271

Anwendungsinformationen

Applikationshinweise:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human COMT standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum and plasma (heparin, EDTA) to each empty well. It is recommended that each human COMT standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 31.2pg/mL-2000pg/mL

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Details zu COMT

Target: COMT (Catechol-O-Methyltransferase (COMT))

Andere Bezeichnung: COMT (COMT Produkte)

Synonyme: Comt1 ELISA Kit, D16Wsu103e ELISA Kit, D330014B15Rik ELISA Kit, COMT ELISA Kit, mb-comt ELISA Kit, s-comt ELISA Kit, comt ELISA Kit, MGC154444 ELISA Kit, zgc:114157 ELISA Kit, si:dkey-13a21.15 ELISA Kit, zK13A21.15 ELISA Kit, zgc:162236 ELISA Kit, catechol-O-methyltransferase ELISA Kit, microRNA 4761 ELISA Kit, catechol-O-methyltransferase S homeolog ELISA Kit, methyltransferase ELISA Kit, catechol-o-methyltransferase ELISA Kit, catechol-O-methyltransferase a ELISA Kit, catechol-O-methyltransferase b ELISA Kit, catechol O-methyltransferase ELISA Kit, COMT ELISA Kit, Comt ELISA Kit, MIR4761 ELISA Kit, comt ELISA Kit, comt.2 ELISA Kit, comt.S ELISA Kit, Rv1703c ELISA Kit, Mb1729c ELISA Kit, comta ELISA Kit, comtb ELISA Kit, LOC100354142 ELISA Kit

Gen-ID: 1312

NCBI Accession: NP_000745

UniProt: P21964

Pathways: Steroid Hormone Biosynthesis, SARS-CoV-2 Protein Interaktom