COL1A1 ELISA Kit

Produktnummer ABIN4882440

Produktdetails COL1A1 ELISA Kit

Target: COL1A1 (Collagen, Type I, alpha 1 (COL1A1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Custom Human Collagen alpha-1 chain ELISA Kit.

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Human LRRN1.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu COL1A1

Target: COL1A1 (Collagen, Type I, alpha 1 (COL1A1))

Andere Bezeichnung: Collagen alpha-1 Chain (COL1A1 Produkte)

Synonyme: OI4 ELISA Kit, Col1a-1 ELISA Kit, Cola-1 ELISA Kit, Cola1 ELISA Kit, Mov-13 ELISA Kit, Mov13 ELISA Kit, COLIA1 ELISA Kit, col1a1 ELISA Kit, MGC52532 ELISA Kit, COL1A1 ELISA Kit, COL1A2 ELISA Kit, alpha1(I) ELISA Kit, cb21 ELISA Kit, sb:cb21 ELISA Kit, sb:cb384 ELISA Kit, wu:fa99c12 ELISA Kit, wu:fb02c06 ELISA Kit, alpha3(I) ELISA Kit, col1a3 ELISA Kit, hm:zeh0348 ELISA Kit, wu:fa95h05 ELISA Kit, wu:fd02a10 ELISA Kit, wu:fj59a10 ELISA Kit, collagen type I alpha 1 chain ELISA Kit, collagen, type I, alpha 1 ELISA Kit, collagen, type I, alpha 1 S homeolog ELISA Kit, collagen alpha-1(I) chain ELISA Kit, collagen type I alpha 2 chain ELISA Kit, collagen, type I, alpha 1a ELISA Kit, collagen 1a1 ELISA Kit, collagen, type I, alpha 1b ELISA Kit, COL1A1 ELISA Kit, Col1a1 ELISA Kit, col1a1.S ELISA Kit, LOC397571 ELISA Kit, COL1A2 ELISA Kit, col1a1a ELISA Kit, col1a1 ELISA Kit, col1a1b ELISA Kit

Gen-ID: 1277

UniProt: P02452

Pathways: Sensory Perception of Sound, Autophagie, Growth Factor Binding