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COL1A1 ELISA Kit (Collagen, Type I, alpha 1) ELISA Kit

COL1A1 Reaktivität: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN4882440
$541.20
Zzgl. Versandkosten $45.00
Lieferung in 31 bis 41 Werktagen
  • Target Alle COL1A1 ELISA Kits anzeigen
    COL1A1
    Reaktivität
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Custom Human Collagen alpha-1 chain ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Spezifität
    This ELISA antibody pair recognizes Human LRRN1.
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Featured
    Zu unserem meistverkauften COL1A1 ELISA Kit
  • Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle COL1A1 ELISA Kits anzeigen
    COL1A1
    Andere Bezeichnung
    Collagen alpha-1 Chain (COL1A1 Produkte)
    Synonyme
    OI4, Col1a-1, Cola-1, Cola1, Mov-13, Mov13, COLIA1, col1a1, MGC52532, COL1A1, COL1A2, alpha1(I), cb21, sb:cb21, sb:cb384, wu:fa99c12, wu:fb02c06, alpha3(I), col1a3, hm:zeh0348, wu:fa95h05, wu:fd02a10, wu:fj59a10, collagen type I alpha 1 chain, collagen, type I, alpha 1, collagen, type I, alpha 1 S homeolog, collagen alpha-1(I) chain, collagen type I alpha 2 chain, collagen, type I, alpha 1a, collagen 1a1, collagen, type I, alpha 1b, COL1A1, Col1a1, col1a1.S, LOC397571, COL1A2, col1a1a, col1a1, col1a1b
    Gen-ID
    1277
    UniProt
    P02452
    Pathways
    Sensory Perception of Sound, Autophagie, Growth Factor Binding
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