Angiotensin I Converting Enzyme 1 ELISA Kit
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- Target Alle Angiotensin I Converting Enzyme 1 (ACE) ELISA Kits anzeigen
- Angiotensin I Converting Enzyme 1 (ACE) (Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE))
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Reaktivität
- Human
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Applikation
- ELISA
- Verwendungszweck
- Custom Human ACE (CD143) ELISA Kit.
- Proben
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
- Analytische Methode
- Quantitative
- Spezifität
- This ELISA antibody pair recognizes human ACE. Other species not yet determined.
- Produktmerkmale
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Bestandteile
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Benötigtes Material
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Top Product
- Discover our top product ACE ELISA Kit
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- Probenmenge
- 100 μL
- Plattentyp
- Pre-coated
- Protokoll
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Lagerung
- -20 °C
- Informationen zur Lagerung
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Haltbarkeit
- 6 months
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- Target Alle Angiotensin I Converting Enzyme 1 (ACE) ELISA Kits anzeigen
- Angiotensin I Converting Enzyme 1 (ACE) (Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE))
- Andere Bezeichnung
- ACE (ACE Produkte)
- Synonyme
- ACE1 ELISA Kit, CD143 ELISA Kit, DCP ELISA Kit, DCP1 ELISA Kit, ICH ELISA Kit, MVCD3 ELISA Kit, AW208573 ELISA Kit, Dcp1 ELISA Kit, StsRR92 ELISA Kit, dcp ELISA Kit, ace1 ELISA Kit, dcp1 ELISA Kit, xace ELISA Kit, cd143 ELISA Kit, ACE ELISA Kit, angiotensin I converting enzyme ELISA Kit, angiotensin I converting enzyme (peptidyl-dipeptidase A) 1 ELISA Kit, angiotensin-converting enzyme ELISA Kit, angiotensin I converting enzyme (peptidyl-dipeptidase A) 3 ELISA Kit, angiotensin-converting enzyme-like ELISA Kit, ACE ELISA Kit, Ace ELISA Kit, ace ELISA Kit, CpipJ_CPIJ009106 ELISA Kit, ACE3 ELISA Kit, LOC101824864 ELISA Kit
- Gen-ID
- 1636
- UniProt
- P12821
- Pathways
- ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour, Smooth Muscle Cell Migration
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