Interferon gamma ELISA Kit

Produktnummer ABIN455496

Das Maus Interferon gamma ELISA Kit (ABIN455496) ist ein Colorimetric ELISA Kit zur Detektion von Maus Interferon gamma.

Kurzübersicht für Interferon gamma ELISA Kit (ABIN455496)

Target: Interferon gamma (IFNG)

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.6-1000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma, Serum

Produktdetails Interferon gamma ELISA Kit

Untere Nachweisgrenze: 15.6 pg/mL

Verwendungszweck: This immunoassay kit allows for the in vitro quantitative determination of mouse IFN- ? concentrations in cell culture supernates, serum, plasma and other biological fluids.

Analytische Methode: Quantitative

Spezifität: This assay recognizes recombinant and natural mouse IFN- ? .

Kreuzreaktivität (Details): No significant cross-reactivity or interference was observed.

Sensitivität: < 3.9 pg/mL
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.

Produktmerkmale: Mus musculus,Mouse,Interferon gamma,IFN-gamma,Ifng

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IFN- γ . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IFN- γ . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain IFN- γ , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a 2 change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of IFN- γ in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Details zu Interferon gamma

Target: Interferon gamma (IFNG)

Andere Bezeichnung: Ifng

Hintergrund: IFN- γ is a dimerized soluble cytokine that is the only member of the type II class of interferons. This interferon was originally called macrophage-activating factor. The IFN- γ monomer consists of a core of six α -helices and an extended unfolded sequence in the C-terminal region. The biologically active dimer is formed by anti-parallel inter-locking of two monomers. Full length of IFN- γ is 143 amino acids. In contrast to interferon- α and interferon- β which can be expressed by all cells, IFN- γ is secreted by T lymphocytes and NK cells only. Also known as immune interferon, IFN- γ is the only Type II interferon. It is serologically distinct from Type I interferons and it is acid-labile, while the type I variants are acid-stable. IFN- γ has antiviral, immunoregulatory, and anti-tumour properties. It alters transcription in up to 30 genes producing a variety of physiological and cellular responses. Activation by IFN- γ is achieved by its interaction with a heterodimeric receptor consisting of IFNGR1 & IFNGR2 (interferon gamma receptors). IFN- γ binding to the receptor activates the JAK-STAT pathway. In addition, IFN- γ activates APCs and promotes Th1 differentiation by upregulating the transcription factor T-bet. IFN- γ is the hallmark cytokine of Th1 cells (Th2 cells produce IL-4). NK cells and [[CD8+ cytotoxic T cell]]s also produce IFN- γ . IFN- γ suppresses osteoclast formation by rapidly degrading the RANK adaptor protein TRAF6 in the RANK-RANKL signaling pathway, which otherwise stimulates the production of NF κ B.

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagie