Cholecystokinin ELISA Kit

Produktnummer ABIN454698

Das Maus Cholecystokinin ELISA Kit (ABIN454698) ist ein Colorimetric ELISA Kit zur Detektion von Maus Cholecystokinin.

Kurzübersicht für Cholecystokinin ELISA Kit (ABIN454698)

Target: Cholecystokinin (CCK)

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Proben: Serum, Tissue Homogenate

Produktdetails Cholecystokinin ELISA Kit

Verwendungszweck: This immunoassay kit allows for the in vitro quantitative determination of Mouse Cholecystokinin,CCK, concentrations in serum, tissue homogenates and other biological fluids.

Analytische Methode: Quantitative

Spezifität: This assay recognizes recombinant and natural mouse CCK.

Kreuzreaktivität (Details): No significant cross-reactivity or interference was observed.

Sensitivität: The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.

Produktmerkmale: Mus musculus,Mouse,Cholecystokinin,CCK,Cck

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CCK. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CCK and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CCK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CCK in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Details zu Cholecystokinin

Target: Cholecystokinin (CCK)

Andere Bezeichnung: Cck

Pathways: T-Zell Rezeptor Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades, Feeding Behaviour