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Albumin ELISA Kit

ALB Reaktivität: Rind (Kuh) Colorimetric Competition ELISA Urine
Produktnummer ABIN454014
  • Target Alle Albumin (ALB) ELISA Kits anzeigen
    Albumin (ALB)
    Reaktivität
    • 10
    • 8
    • 7
    • 6
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    Rind (Kuh)
    Nachweismethode
    Colorimetric
    Methodentyp
    Competition ELISA
    Untere Nachweisgrenze
    50 ng/mL
    Applikation
    ELISA
    Verwendungszweck
    The ALBUMIN(bovine)-ELISA is designed for the quantitative determination of bovine albumin in complex samples.
    Proben
    Urine
    Analytische Methode
    Quantitative
    Spezifität
    Albumin (human) <0.1%Albumin (mouse) <0.1%
    Produktmerkmale
    The determination of albumin is carried out as direct competitive ELISA. Main application of the assay is to monitor purification of protein preparations (e.g. monoclonal antibodies) if bovine or calf serum albumin has been used in the production process. This ELISA furthermore allows quantification of albumin in bovine urine to estimate kidney function. When blood is filtered in glomeruli of kidneys, highly molecular substances are held back. Therefore, urine is nearly protein free. In case of kidney damages, small proteins like albumin appear in urine. Albumin therefore serves as a marker in monitoring kidney damages.
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  • Plattentyp
    Pre-coated
    Protokoll
    Bovine albumin has been pre-coated onto a microplate. During incubation the binding of an enzyme-linked anti-albumin(bovine)-antibody to the wells is inhibited by albumin from samples/standards. After washing away any unbound antibody, a substrate solution is added to the wells and color develops in proportion to the amount of antibody conjugate. The absorption at 450 nm is reverse proportional to the albumin concentration.
    Testdurchführung

    Incubation of samples/standards with antibody conjugate: 50 µL + 50 µL/well 2 hrs, room temperature.
    Wash Substrate incubation: 100 µL/well 20 min, room temperature.
    Add stop solution: 100 µL/well.
    Read at 450 nm

    Testpräzision
    Intraassay precision (CV):(n=10)at 2529 ng/ml: 5.1%at 875 ng/ml: 8.8%at 39 ng/ml: 10.7% Recovery:106.6%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C
  • Target Alle Albumin (ALB) ELISA Kits anzeigen
    Albumin (ALB)
    Andere Bezeichnung
    Albumin (ALB Produkte)
    Synonyme
    PRO0883 ELISA Kit, PRO0903 ELISA Kit, PRO1341 ELISA Kit, ALB ELISA Kit, CSA ELISA Kit, Alb-1 ELISA Kit, Alb1 ELISA Kit, Albza ELISA Kit, LOC100136344 ELISA Kit, alb-a ELISA Kit, alb-b ELISA Kit, albumin ELISA Kit, serum albumin 1 ELISA Kit, albumin S homeolog ELISA Kit, ALB ELISA Kit, Alb ELISA Kit, LOC100136344 ELISA Kit, alb.S ELISA Kit
    Pathways
    Lipid Metabolism
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