Albumin ELISA Kit

Produktnummer ABIN454014

Dieses Rind (Kuh) Albumin ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Rind (Kuh) Albumin zu quantifizieren.

Kurzübersicht für Albumin ELISA Kit (ABIN454014)

Target: Albumin (ALB)

Reaktivität: Rind (Kuh)

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Applikation: ELISA

Proben: Urine

Produktdetails Albumin ELISA Kit

Untere Nachweisgrenze: 50 ng/mL

Verwendungszweck: The ALBUMIN(bovine)-ELISA is designed for the quantitative determination of bovine albumin in complex samples.

Analytische Methode: Quantitative

Spezifität: Albumin (human) <0.1%Albumin (mouse) <0.1%

Produktmerkmale: The determination of albumin is carried out as direct competitive ELISA. Main application of the assay is to monitor purification of protein preparations (e.g. monoclonal antibodies) if bovine or calf serum albumin has been used in the production process. This ELISA furthermore allows quantification of albumin in bovine urine to estimate kidney function. When blood is filtered in glomeruli of kidneys, highly molecular substances are held back. Therefore, urine is nearly protein free. In case of kidney damages, small proteins like albumin appear in urine. Albumin therefore serves as a marker in monitoring kidney damages.

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Bovine albumin has been pre-coated onto a microplate. During incubation the binding of an enzyme-linked anti-albumin(bovine)-antibody to the wells is inhibited by albumin from samples/standards. After washing away any unbound antibody, a substrate solution is added to the wells and color develops in proportion to the amount of antibody conjugate. The absorption at 450 nm is reverse proportional to the albumin concentration.

Testdurchführung:

Incubation of samples/standards with antibody conjugate: 50 µL + 50 µL/well 2 hrs, room temperature.
Wash Substrate incubation: 100 µL/well 20 min, room temperature.
Add stop solution: 100 µL/well.
Read at 450 nm

Testpräzision: Intraassay precision (CV):(n=10)at 2529 ng/ml: 5.1%at 875 ng/ml: 8.8%at 39 ng/ml: 10.7% Recovery:106.6%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Details zu Albumin

Target: Albumin (ALB)

Andere Bezeichnung: Albumin

Pathways: Lipid Metabolism