SFRP5 ELISA Kit

Produktnummer ABIN418512

Das Human SFRP5 ELISA Kit (ABIN418512) ist ein Colorimetric ELISA Kit zur Detektion von Human SFRP5. Das Kit wurde in 20 Publikationen zitiert.

Kurzübersicht für SFRP5 ELISA Kit (ABIN418512)

Target: SFRP5 (Secreted Frizzled-Related Protein 5 (SFRP5))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56 ng/mL - 100 ng/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Produktdetails SFRP5 ELISA Kit

Untere Nachweisgrenze: 1.56 ng/mL

Verwendungszweck: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of SFRP5 in Serum,Plasma,Biological Fluids

Analytische Methode: Quantitative

Spezifität:

This assay has high sensitivity and excellent specificity for detection of Secreted Frizzled Related Protein 5 (SFRP5).
No significant cross-reactivity or interference between Secreted Frizzled Related Protein 5 (SFRP5) and analogues was observed.

Kreuzreaktivität (Details): No significant cross-reactivity or interference between Secreted Frizzled Related Protein 5 (SFRP5) and analogues was observed.

Sensitivität: < 0.58 ng/mL

Bestandteile:

• Pre-coated, 96-well strip plate
• Plate sealer
• Standard
• Standard Diluent
• Detection Reagent A
• Assay Diluent A
• Detection Reagent B
• Assay Diluent B
• TMB Substrate
• Stop Solution
• Wash Buffer (30X)
• Instruction manual

Benötigtes Material:

• Microplate reader with 450 nm filter.
• Precision single or multi-channel pipettes and disposable tips.
• Eppendorf Tubes for diluting samples.
• Deionized or distilled water.
• Absorbent paper for blotting the microtiter plate.
• Container for Wash Solution

Anwendungsinformationen

Applikationshinweise:

• Limited by the current condition and scientific technology, we cannot completely conduct the comprehensive identification and analysis on the raw material provided by suppliers. So there might be some qualitative and technical risks to use the kit.
• The final experimental results will be closely related to validity of the products, operation skills of the end users and the experimental environments. Please make sure that sufficient samples are available.
• Kits from different batches may be a little different in detection range, sensitivity and color developing time.
• Do not mix or substitute reagents from one kit lot to another. Use only the reagents supplied by manufacturer.
• Protect all reagents from strong light during storage and incubation. All the bottle caps of reagents should be covered tightly to prevent the evaporation and contamination of microorganism.
• There may be some foggy substance in the wells when the plate is opened at the first time. It will not have any effect on the final assay results. Do not remove microtiter plate from the storage bag until needed.
• Wrong operations during the reagents preparation and loading, as well as incorrect parameter setting for the plate reader may lead to incorrect results. A microplate plate reader with a bandwidth of 10nm or less and an optical density range of 0-3 O.D. or greater at 450 ± 10nm wavelength is acceptable for use in absorbance measurement. Please read the instruction carefully and adjust the instrument prior to the experiment.
• Even the same operator might get different results in two separate experiments. In order to get better reproducible results, the operation of every step in the assay should be controlled. Furthermore, a preliminary experiment before assay for each batch is recommended.
• Each kit has been strictly passed Q.C test. However, results from end users might be inconsistent with our in-house data due to some unexpected transportation conditions or different lab equipments. Intra-assay variance among kits from different batches might arise from above factors, too.
• Kits from different manufacturers for the same item might produce different results, since we have not compared our products with other manufacturers.

Kommentare:

Information on standard material:
The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

Information on reagents:
The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

Information on antibodies:
The provided antibodies and their host vary in different kits.

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

Testpräzision:

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Secreted Frizzled Related Protein 5 (SFRP5) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Secreted Frizzled Related Protein 5 (SFRP5) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Vorsichtsmaßnahmen: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Handhabung: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Lagerung: 4 °C

Informationen zur Lagerung:

• For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
• For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
  Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.
• For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.

Haltbarkeit: 6 months

Referenzen für SFRP5 ELISA Kit (ABIN418512)

Song, Zou, Song, Xu, Qian, Zhu, Liu, Guan, Chen, Yi: "Association of Adipocytokines With Carotid Intima Media Thickness and Arterial Stiffness in Obstructive Sleep Apnea Patients." in: Frontiers in endocrinology, Vol. 11, pp. 177, (2020) (PubMed).

Báez, Castro, Gutiérrez, López, Lemarroy, Suarez, Ureta, Montoya, Andalón et al.: "Association of serum levels of secreted frizzled-related protein 5 and Wnt member 5a with glomerular filtration rate in patients with type 2 diabetes mellitus and chronic renal disease: a ..." in: Sao Paulo medical journal = Revista paulista de medicina, Vol. 138, Issue 2, pp. 133-139, (2020) (PubMed).

Koebnick, Black, Wu, Shu, MacKay, Watanabe, Buchanan, Xiang: "A diet high in sugar-sweetened beverage and low in fruits and vegetables is associated with adiposity and a pro-inflammatory adipokine profile." in: The British journal of nutrition, Vol. 120, Issue 11, pp. 1230-1239, (2019) (PubMed).

Carstensen-Kirberg, Kannenberg, Huth, Meisinger, Koenig, Heier, Peters, Rathmann, Roden, Herder, Thorand: "Inverse associations between serum levels of secreted frizzled-related protein-5 (SFRP5) and multiple cardiometabolic risk factors: KORA F4 study." in: Cardiovascular diabetology, Vol. 16, Issue 1, pp. 109, (2018) (PubMed).

Black, Shu, Wu, Koebnick, MacKay, Watanabe, Buchanan, Xiang: "Longitudinal Increases in Adiposity Contribute to Worsening Adipokine Profile over Time in Mexican Americans." in: Obesity (Silver Spring, Md.), Vol. 26, Issue 4, pp. 703-712, (2018) (PubMed).

Cho, Kang, Lee, Lee, Seol, Lee, Park, Jung: "Effect of SFRP5 (Secreted Frizzled-Related Protein 5) on the WNT5A (Wingless-Type Family Member 5A)-Induced Endothelial Dysfunction and Its Relevance With Arterial Stiffness in Human Subjects." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 38, Issue 6, pp. 1358-1367, (2018) (PubMed).

Xu, Wang, Li, Wang, Lai, Gao, Lei, Yang, Liao, Fang, Liu, Li: "Plasma Sfrp5 levels correlate with determinants of the metabolic syndrome in Chinese adults." in: Diabetes/metabolism research and reviews, Vol. 33, Issue 6, (2017) (PubMed).

Oztas, Ozler, Ersoy, Ersoy, Tokmak, Ergin, Uygur, Danisman: "Prediction of gestational diabetes mellitus by first trimester serum secreted frizzle-related protein-5 levels." in: The journal of maternal-fetal & neonatal medicine, Vol. 29, Issue 9, pp. 1515-9, (2016) (PubMed).

Carstensen-Kirberg, Hatziagelaki, Tsiavou, Chounta, Nowotny, Pacini, Dimitriadis, Roden, Herder: "Sfrp5 associates with beta-cell function in humans." in: European journal of clinical investigation, Vol. 46, Issue 6, pp. 535-43, (2016) (PubMed).

Chen, He, Wu, Dai, Zhao, Huang, Jiang: "Bone marrow sFRP5 level is negatively associated with bone formation markers." in: Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA, Vol. 28, Issue 4, pp. 1305-1311, (2016) (PubMed).

Canivell, Rebuffat, G Ruano, Kostov, Sisó-Almirall, Novials, Ceriello, Gomis: "Circulating SFRP5 levels are elevated in drug-naïve recently diagnosed type 2 diabetic patients as compared with prediabetic subjects and controls." in: Diabetes/metabolism research and reviews, Vol. 31, Issue 2, pp. 212-9, (2015) (PubMed).

Schulte, Kragelund, Müller, Hagen, Elke, Titz, Schädler, Schumacher, Weiler, Bewig, Schreiber, Laudes: "The wingless-related integration site-5a/secreted frizzled-related protein-5 system is dysregulated in human sepsis." in: Clinical and experimental immunology, Vol. 180, Issue 1, pp. 90-7, (2015) (PubMed).

Tan, Wang, Chu, Liu, Yi, Xiao: "SFRP5 correlates with obesity and metabolic syndrome and increases after weight loss in children." in: Clinical endocrinology, Vol. 81, Issue 3, pp. 363-9, (2014) (PubMed).

Prats-Puig, Soriano-Rodríguez, Carreras-Badosa, Riera-Pérez, Ros-Miquel, Gomila-Borja, de Zegher, Ibáñez, Bassols, López-Bermejo: "Balanced duo of anti-inflammatory SFRP5 and proinflammatory WNT5A in children." in: Pediatric research, Vol. 75, Issue 6, pp. 793-7, (2014) (PubMed).

Catalán, Gómez-Ambrosi, Rodríguez, Pérez-Hernández, Gurbindo, Ramírez, Méndez-Giménez, Rotellar, Valentí, Moncada, Martí, Sola, Silva, Salvador, Frühbeck: "Activation of noncanonical Wnt signaling through WNT5A in visceral adipose tissue of obese subjects is related to inflammation." in: The Journal of clinical endocrinology and metabolism, Vol. 99, Issue 8, pp. E1407-17, (2014) (PubMed).

Peng, Xiao, Kang, He, Li: "Serum secreted frizzled-related protein 5 levels differentially decrease in patients with hepatitis B virus-associated chronic infection and hepatocellular carcinoma." in: Oncology letters, Vol. 8, Issue 3, pp. 1340-1344, (2014) (PubMed).

Flehmig, Scholz, Klöting, Fasshauer, Tönjes, Stumvoll, Youn, Blüher: "Identification of adipokine clusters related to parameters of fat mass, insulin sensitivity and inflammation." in: PLoS ONE, Vol. 9, Issue 6, pp. e99785, (2014) (PubMed).

Hu, Deng, Qu: "Plasma SFRP5 levels are decreased in Chinese subjects with obesity and type 2 diabetes and negatively correlated with parameters of insulin resistance." in: Diabetes research and clinical practice, Vol. 99, Issue 3, pp. 391-5, (2013) (PubMed).

Carstensen, Herder, Kempf, Erlund, Martin, Koenig, Sundvall, Bidel, Kuha, Roden, Tuomilehto: "Sfrp5 correlates with insulin resistance and oxidative stress." in: European journal of clinical investigation, Vol. 43, Issue 4, pp. 350-7, (2013) (PubMed).

Schulte, Müller, Neumann, Oberhäuser, Faust, Güdelhöfer, Brandt, Krone, Laudes: "Pro-inflammatory wnt5a and anti-inflammatory sFRP5 are differentially regulated by nutritional factors in obese human subjects." in: PLoS ONE, Vol. 7, Issue 2, pp. e32437, (2012) (PubMed).

Details zu SFRP5

Target: SFRP5 (Secreted Frizzled-Related Protein 5 (SFRP5))

Andere Bezeichnung: SFRP5

Hintergrund: SARP3, Secreted Apoptosis Related Protein 3, Frizzled-related protein 1b

UniProt: Q5T4F7

Pathways: WNT Signalweg