PAI1 ELISA Kit

Produktnummer ABIN411404

Produktdetails PAI1 ELISA Kit

Target: PAI1 (SERPINE1) (Plasminogen Activator Inhibitor 1 (SERPINE1))

Bindungsspezifität: AA 22-402

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156-10000 pg/mL

Untere Nachweisgrenze: 156 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human PAI-1

Marke: PicoKine™

Proben: Cell Culture Supernatant, Cell Lysate, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)

Analytische Methode: Quantitative

Spezifität: Expression system for standard: sf21
Immunogen sequence: S22-P402

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: sf21
Immunogen sequence: S22-P402

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Sequence similarities: Belongs to the serpin family.

Tissue Specificity: Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.

Plattentyp: Pre-coated

Protokoll: human PAI-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for PAI-1 has been precoated onto 96-well plates. Standards(sf21, S22-P402) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PAI-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human PAI-1 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human PAI-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, serum or plasma(heparin, EDTA, citrate ) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human PAI-1 standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 1539, Standard deviation: 76.95, CV(%): 5
• Sample 2: n=16, Mean(pg/ml): 3736, Standard deviation: 235.4, CV(%): 6.3
• Sample 3: n=16, Mean(pg/ml): 6234, Standard deviation: 405.21, CV(%): 6.5,
• Sample 1: n=24, Mean(pg/ml): 1637, Standard deviation: 104.8, CV(%): 6.4
• Sample 2: n=24, Mean(pg/ml): 3914, Standard deviation: 293.55, CV(%): 7.5
• Sample 3: n=24, Mean(pg/ml): 7335, Standard deviation: 601.47, CV(%): 8.2

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Referenzen für PAI1 ELISA Kit (ABIN411404)

He, Le, Frediani, Winterberg, Jin, Liverman, Hernandez, Cleeton, Vos: "Cardiometabolic risks vary by weight status in pediatric kidney and liver transplant recipients: A cross-sectional, single-center study in the USA." in: Pediatric transplantation, Vol. 21, Issue 6, (2018) (PubMed).

Sun, Yang, Xi, Chen, Fu, Sun, Yin, Li, Liu, Qin, Liu, Hou: "Primed atypical ductal hyperplasia-associated fibroblasts promote cell growth and polarity changes of transformed epithelium-like breast cancer MCF-7 cells via miR-200b/c-IKKβ signaling." in: Cell death & disease, Vol. 9, Issue 2, pp. 122, (2018) (PubMed).

Sun, Song, Liu, Fang, Wang, Wang, Li, Tang, Wu, Qin, Wu, Gao, Chen, Hu: "Associations between homocysteine metabolism related SNPs and carotid intima-media thickness: a Chinese sib pair study." in: Journal of thrombosis and thrombolysis, Vol. 43, Issue 3, pp. 401-410, (2017) (PubMed).

Nargesi, Shalchi, Nargesi, Sadeghpour, Zarifkar, Mozaffari, Imani, Esteghamati, Nakhjavani: "The lost correlation between heat shock protein 70 (HSPA1A) and plasminogen activator inhibitor-1 in patients with type 2 diabetes and albuminuria." in: Cell stress & chaperones, Vol. 21, Issue 2, pp. 361-5, (2016) (PubMed).

Wu, Liu, Chen, Chen, Zhang: "Disequilibrium of Blood Coagulation and Fibrinolytic System in Patients With Coronary Artery Ectasia." in: Medicine, Vol. 95, Issue 8, pp. e2779, (2016) (PubMed).

Chen, Lu, Yang, Lu, Chen, Zhang: "Suppression of plasminogen activator inhibitor-1 by inhaled nitric oxide attenuates the adverse effects of hyperoxia in a rat model of acute lung injury." in: Thrombosis research, Vol. 136, Issue 1, pp. 131-8, (2015) (PubMed).

Details zu PAI1

Target: PAI1 (SERPINE1) (Plasminogen Activator Inhibitor 1 (SERPINE1))

Andere Bezeichnung: Plasminogen activator inhibitor-1 (PAI-1) (SERPINE1 Produkte)

Synonyme: PAI ELISA Kit, PAI-1 ELISA Kit, PAI1 ELISA Kit, PLANH1 ELISA Kit, SERPINE1 ELISA Kit, si:ch211-138a11.1 ELISA Kit, Planh1 ELISA Kit, PAI1A ELISA Kit, Pai1 ELISA Kit, Pai1aa ELISA Kit, Planh ELISA Kit, RATPAI1A ELISA Kit, serpin family E member 1 ELISA Kit, serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 L homeolog ELISA Kit, serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 ELISA Kit, serine (or cysteine) peptidase inhibitor, clade E, member 1 ELISA Kit, SERPINE1 ELISA Kit, serpine1.L ELISA Kit, serpine1 ELISA Kit, Serpine1 ELISA Kit

Hintergrund:

Protein Function: Serine protease inhibitor. This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, protein C and matriptase-3/TMPRSS7. Its rapid interaction with PLAT may function as a major control point in the regulation of fibrinolysis. .

Background: Plasminogen activator inhibitor-1 is the principal inhibitor of tissue plasminogen activator(tPA) and urokinase(uPA), the activators of plasminogen and hence fibrinolysis(the physiological breakdown of blood clots). It is a serine protease inhibitor(serpin) protein(SERPINE1). PAI-1 is mainly produced by the endothelium(cells lining blood vessels), but is also secreted by other tissue types, such as adipose tissue. The PAI-1 gene is located on the seventh chromosome(7q21.3-q22).1 Congenital deficiency of PAI-1 leads to a hemorrhagic diathesis(a tendency to hemorrhage). PAI-1 is present in increased levels in various disease states(such as a number of forms of cancer), as well as in obesity and the metabolic syndrome. It has been linked to the increased occurrence of thrombosis in patients with these conditions. Additionally, it has been shown to interact with ORM1.2 The standard product used in this kit is recombinant human PAI-1 with the molecular mass of 43KDa.

Synonyms: Plasminogen activator inhibitor 1,PAI,PAI-1,Endothelial plasminogen activator inhibitor,Serpin E1,SERPINE1,PAI1, PLANH1,

Full Gene Name: Plasminogen activator inhibitor 1

Cellular Localisation: Secreted.

Gen-ID: 5054

UniProt: P05121

Pathways: p53 Signalweg, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagie, Smooth Muscle Cell Migration