Thrombospondin 2 ELISA Kit

Produktnummer ABIN411388

Human Thrombospondin 2 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human Thrombospondin 2.

Kurzübersicht für Thrombospondin 2 ELISA Kit (ABIN411388)

Target: Thrombospondin 2 (THBS2)

Bindungsspezifität: AA 19-1172

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156-10000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Milk

Produktdetails Thrombospondin 2 ELISA Kit

Untere Nachweisgrenze: 156 pg/mL

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TSP2

Marke: PicoKine™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NSO,G19-I1172

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: G19-I1172

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Sequence similarities: Belongs to the thrombospondin family.

Tissue Specificity: High expression in invertebral disk tissue. .

Plattentyp: Pre-coated

Protokoll: human TSP2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TSP2 has been precoated onto 96-well plates. Standards (NSO,G19-I1172) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TSP2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TSP2 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human TSP2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma( heparin, EDTA) or human milk to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human TSP2 standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(ng/ml): 0.56, Standard deviation: 0.04, CV(%): 7.1
• Sample 2: n=16, Mean(ng/ml): 1.29, Standard deviation: 0.08, CV(%): 6.2
• Sample 3: n=16, Mean(ng/ml): 5.84, Standard deviation: 0.32, CV(%): 5.5,
• Sample 1: n=24, Mean(ng/ml): 0.62, Standard deviation: 0.05, CV(%): 8.1
• Sample 2: n=24, Mean(ng/ml): 1.36, Standard deviation: 0.1, CV(%): 7.4
• Sample 3: n=24, Mean(ng/ml): 6.47, Standard deviation: 0.42, CV(%): 6.5

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Details zu Thrombospondin 2

Target: Thrombospondin 2 (THBS2)

Andere Bezeichnung: THBS2

Hintergrund:

Protein Function: Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Ligand for CD36 mediating antiangiogenic properties. .

Background: Thrombospondin-2(TSP-2) is a protein that in humans is encoded by the THBS2 gene.And this gene is mapped to 6q27. The protein encoded by this gene belongs to the thrombospondin family. It is a disulfide-linked homotrimeric glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein has been shown to function as a potent inhibitor of tumor growth and angiogenesis. Studies of the mouse counterpart suggest that this protein may modulate the cell surface properties of mesenchymal cells and be involved in cell adhesion and migration. TSP-2 has been shown to interact with MMP2.

Synonyms: Thrombospondin-2,THBS2,TSP2,

Full Gene Name: Thrombospondin-2

Gen-ID: 7058

UniProt: P35442