Thrombospondin 2 ELISA Kit

Produktnummer ABIN6720120

Hersteller Produkt- Nr.: koa0370

Das Human Thrombospondin 2 ELISA Kit (ABIN6720120) ist ein Colorimetric ELISA Kit zur Detektion von Human Thrombospondin 2.

Kurzübersicht für Thrombospondin 2 ELISA Kit (ABIN6720120)

Target: Thrombospondin 2 (THBS2)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 625 pg/mL - 40000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Milk, Plasma (EDTA), Plasma (heparin), Serum

Produktdetails Thrombospondin 2 ELISA Kit

Untere Nachweisgrenze: 625 pg/mL

Hersteller Produkt- Nr.: koa0370

Hersteller: Rockland

Verwendungszweck: Human TSP2 Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NS0
Immunogen sequence: G19-I1172

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Benötigtes Material: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Anwendungsinformationen

Kommentare:

Capture antibody and Detection antibody: polyclonal antibody from goat, polyclonal antibody from goat

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human TSP2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma( heparin, EDTA) or human milk to each empty well. It is recommended that each human TSP2 standard solution and each sample be measured in duplicate.

Testpräzision: Intra-Assay Precision (CV%): 7.5%, 4.6%, 6.8%
Inter-Assay Precision (CV%): 8%, 6.5%, 7.7%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu Thrombospondin 2

Target: Thrombospondin 2 (THBS2)

Andere Bezeichnung: TSP2

Hintergrund:

Background: Thrombospondin-2 (TSP-2) is a protein that in humans is encoded by the THBS2 gene.And this gene is mapped to 6q27. The protein encoded by this gene belongs to the thrombospondin family. It is a disulfide-linked homotrimeric glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein has been shown to function as a potent inhibitor of tumor growth and angiogenesis. Studies of the mouse counterpart suggest that this protein may modulate the cell surface properties of mesenchymal cells and be involved in cell adhesion and migration. TSP-2 has been shown to interact with MMP2.

Gene Full Name: thrombospondin 2

Gen-ID: 7058

NCBI Accession: NP_003238

UniProt: P35442