VEGFC ELISA Kit

Produktnummer ABIN411382

Human VEGFC ELISA Kit, Colorimetric Assay für die Quantifizierung von Human VEGFC und es wurde in 10+ Publikationen zitiert.

Kurzübersicht für VEGFC ELISA Kit (ABIN411382)

Target: VEGFC (Vascular Endothelial Growth Factor C (VEGFC))

Bindungsspezifität: AA 103-227

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5 pg/mL - 4000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Produktdetails VEGFC ELISA Kit

Untere Nachweisgrenze: 62.5 pg/mL

Verwendungszweck: Sandwich Human VEGF-C ELISA Kit to quantitate Human VEGFC in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NS0
Immunogen sequence: T103-R227

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 3 pg/mL

Bestandteile:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Benötigtes Material: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Testpräzision: Intra-Assay Precision (CV%): 4.7%, 6.7%, 5.4%
Inter-Assay Precision (CV%): 6.2%, 8.6%, 5.7%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Haltbarkeit: 12 months

Referenzen für VEGFC ELISA Kit (ABIN411382)

Dai, Cui, Zhang, Cheng, Liu, Yang, Fan, Wang, Lin, Zhang, Li, Mao, Wang, Su, Zhang, Peng, Yang, Hu, Yang, Huang, Xiang, Yu, Zhou, Wei, Deng: "SARI inhibits angiogenesis and tumour growth of human colon cancer through directly targeting ceruloplasmin." in: Nature communications, Vol. 7, pp. 11996, (2018) (PubMed).

Zhu, Huang, Huang, Zheng, Hua, Yang, Zhuang, Wang, Ye: "Lipopolysaccharide increases the release of VEGF-C that enhances cell motility and promotes lymphangiogenesis and lymphatic metastasis through the TLR4- NF-κB/JNK pathways in colorectal cancer." in: Oncotarget, Vol. 7, Issue 45, pp. 73711-73724, (2018) (PubMed).

Xu, Ge, Han, Yang, Su, Zhao, Zhao, Yang, Yang: "High-mobility group box 1 expression and lymph node metastasis in intrahepatic cholangiocarcinoma." in: World journal of gastroenterology : WJG, Vol. 21, Issue 11, pp. 3256-65, (2015) (PubMed).

Li, Wang, Zhao, Yang, Li, Zhao, Yu, Wang, Meng, Liu, Zhu, Gao, Jia, Yang: "Nuclear factor of activated T cells 5 maintained by Hotair suppression of miR-568 upregulates S100 calcium binding protein A4 to promote breast cancer metastasis." in: Breast cancer research : BCR, Vol. 16, Issue 5, pp. 454, (2015) (PubMed).

Song, Yue, Li, Li, Zhao, Zhang: "Study of the mechanism of sonodynamic therapy in a rat glioma model." in: OncoTargets and therapy, Vol. 7, pp. 1801-10, (2014) (PubMed).

Zhang, Ding, Diao, Yan, Sun, Hu: "CYR61 modulates the vascular endothelial growth factor C expression of decidual NK cells via PI3K/AKT pathway." in: American journal of reproductive immunology (New York, N.Y. : 1989), Vol. 67, Issue 3, pp. 216-23, (2012) (PubMed).

Chuangui, Peng, Zhentao: "The expression of high mobility group box 1 is associated with lymph node metastasis and poor prognosis in esophageal squamous cell carcinoma." in: Pathology oncology research : POR, Vol. 18, Issue 4, pp. 1021-7, (2012) (PubMed).

Pan, Ren, Ma, Liu, Yu, Ji, Pan, Li, Yang, Lv, Shen, Chen, Zhang, Willard, He, Zheng: "High-density lipoprotein of patients with type 2 diabetes mellitus elevates the capability of promoting migration and invasion of breast cancer cells." in: International journal of cancer. Journal international du cancer, Vol. 131, Issue 1, pp. 70-82, (2012) (PubMed).

Yang, Shi, Li, Yi: "Effects of shRNA targeting maspin on invasion of gastric carcinoma SGC7901 cell line." in: Oncology reports, Vol. 25, Issue 1, pp. 259-65, (2010) (PubMed).

Wang, Zhao, Guo, Zhang, Xie, Xiang, Gao, Wang, Chen: "The expression of RNA-binding protein HuR in non-small cell lung cancer correlates with vascular endothelial growth factor-C expression and lymph node metastasis." in: Oncology, Vol. 76, Issue 6, pp. 420-9, (2009) (PubMed).

Details zu VEGFC

Target: VEGFC (Vascular Endothelial Growth Factor C (VEGFC))

Andere Bezeichnung: VEGFC

Hintergrund:

Background: Vascular endothelial growth factor C is a VEGF. The human gene encoding it is VEGFC. The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family, is active in angiogenesis, lymphangiogenesis and endothelial cell growth and survival, and can also affect the permeability of blood vessels. This secreted protein undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-3 receptors. Only the fully processed form can bind and activate VEGFR-2 receptors. This protein is structurally and functionally similar to vascular endothelial growth factor D (VEGF-D). The C terminus of VEGFC has cysteine-rich repeat units characteristic of the Balbiani ring 3 protein (BR3P) of the midge Chironomus tentans.1,2 The standard product used in this kit is recombinant human VEGF-C, consisting of 135 amino acids with the molecular mass of 23Kda after glycosylation.

Gene Full Name: vascular endothelial growth factor C

Gen-ID: 7424

UniProt: P49767

Pathways: RTK Signalweg, Signaling Events mediated by VEGFR1 and VEGFR2