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Cardiotrophin 1 ELISA Kit

Dieses Human Cardiotrophin 1 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human Cardiotrophin 1 zu quantifizieren.
Produktnummer ABIN411375

Kurzübersicht für Cardiotrophin 1 ELISA Kit (ABIN411375)

Target

Alle Cardiotrophin 1 (CTF1) ELISA Kits anzeigen
Cardiotrophin 1 (CTF1)

Bindungsspezifität

AA 2-201

Reaktivität

  • 6
  • 5
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Human

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Detektionsbereich

156-10000 pg/mL

Applikation

ELISA

Proben

Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
  • Untere Nachweisgrenze

    156 pg/mL

    Verwendungszweck

    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Cardiotrophin-1

    Marke

    PicoKine™

    Analytische Methode

    Quantitative

    Spezifität

    Expression system for standard: E.coli
    Immunogen sequence: S2-A201

    Kreuzreaktivität (Details)

    There is no detectable cross-reactivity with other relevant proteins.

    Sensitivität

    <10pg/mL

    Benötigtes Material

    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

    Immunogen

    Expression system for standard: E.coli
    Immunogen sequence: S2-A201
  • Applikationshinweise

    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

    Kommentare

    Tissue Specificity: Highly expressed in heart, skeletal muscle, prostate and ovary. Lower levels in lung, kidney, pancreas, thymus, testis and small intestine. Little or no expression in brain, placenta, liver, spleen, colon or peripheral blood leukocytes.

    Plattentyp

    Pre-coated

    Protokoll

    human CT-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CT-1 has been precoated onto 96-well plates. Standards(E.coli, S2-A201) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CT-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CT-1 amount of sample captured in plate.

    Testdurchführung

    Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human CT-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CT-1 standard solution and each sample be measured in duplicate.

    Testpräzision

    • Sample 1: n=16, Mean(pg/ml): 253, Standard deviation: 11.38, CV(%): 4.5
    • Sample 2: n=16, Mean(pg/ml): 759, Standard deviation: 35.67, CV(%): 4.7
    • Sample 3: n=16, Mean(pg/ml): 2042, Standard deviation: 98.09, CV(%): 4.9,
    • Sample 1: n=24, Mean(pg/ml): 264, Standard deviation: 12.14, CV(%): 4.6
    • Sample 2: n=24, Mean(pg/ml): 788, Standard deviation: 37.82, CV(%): 4.8
    • Sample 3: n=24, Mean(pg/ml): 1946, Standard deviation: 97.30, CV(%): 5.0

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Handhabung

    Avoid multiple freeze-thaw cycles.

    Lagerung

    -20 °C,4 °C

    Informationen zur Lagerung

    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

    Haltbarkeit

    12 months
  • Target Alle Cardiotrophin 1 (CTF1) ELISA Kits anzeigen

    Cardiotrophin 1 (CTF1)

    Andere Bezeichnung

    CTF1

    Hintergrund

    Protein Function: Induces cardiac myocyte hypertrophy in vitro. Binds to and activates the ILST/gp130 receptor.

    Background: Cardiotrophin-1(CT-1) is a member of the family of cytokines that includes leukemia inhibitory factor(LIF), ciliary neurotrophic factor(CNTF), oncostatin M(OSM), interleukin-6(IL6), and interleukin-11(IL11). And the CT-1 gene is mapped to 1p21-p13. The human CT-1 protein contains 201 amino acids and shares 80 % amino acid identity with the 203-amino acid mouse CT-1 sequence, however, unlike the mouse protein, human CT-1 has 2 rather than 1 cys and has no N-glycosylation site. Despite lacking a signal sequence, secreted CT-1 and mouse CT-1 induce cardiac myocyte hypertrophy in cell culture and bind to both mouse and human LIFR but not to OSMR. Furthermore, A 1.7-kb CT-1 transcript was detected at high levels in heart, skeletal muscle, prostate, and ovary. Low levels were detected in lung, kidney, pancreas, thymus, testis, and small intestine, with little or no expression detected in brain, placenta, spleen, colon, and peripheral blood leukocytes. And it was also observed strong expression in fetal lung and kidney.

    Synonyms: Cardiotrophin-1,CT-1,CTF1,

    Full Gene Name: Cardiotrophin-1

    Cellular Localisation: Secreted.

    Gen-ID

    1489

    UniProt

    Q16619

    Pathways

    JAK-STAT Signalweg
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