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Neurotrophin 3 ELISA Kit

Dieses Maus Neurotrophin 3 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Maus Neurotrophin 3 zu quantifizieren. Dieses ELISA Kit wurde in 2+ Publikationen zitiert.
Produktnummer ABIN411339

Kurzübersicht für Neurotrophin 3 ELISA Kit (ABIN411339)

Target

Alle Neurotrophin 3 (NTF3) ELISA Kits anzeigen
Neurotrophin 3 (NTF3)

Bindungsspezifität

AA 139-257

Reaktivität

  • 6
  • 4
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Maus

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Detektionsbereich

15.6-1000 pg/mL

Applikation

ELISA

Proben

Cell Culture Supernatant, Serum
  • Untere Nachweisgrenze

    15.6 pg/mL

    Verwendungszweck

    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Neurotrophin-3

    Marke

    PicoKine™

    Analytische Methode

    Quantitative

    Spezifität

    Expression system for standard: sf21
    Immunogen sequence: Y139-T257

    Kreuzreaktivität (Details)

    There is cross-reactivity with NT-4<2.5 % , and no detectable cross-reactivity with any other cytokine.

    Homologie

    Bovine,Canine,Chicken,Horse,Monkey,Rabbit

    Sensitivität

    <2pg/mL

    Benötigtes Material

    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

    Immunogen

    Expression system for standard: sf21
    Immunogen sequence: Y139-T257
  • Applikationshinweise

    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

    Kommentare

    Tissue Specificity: Brain and peripheral tissues.

    Plattentyp

    Pre-coated

    Protokoll

    mouse Neurotrophin-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Neurotrophin-3 has been precoated onto 96-well plates. Standards(sf21, Y139-T257) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Neurotrophin-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Neurotrophin-3 amount of sample captured in plate.

    Testdurchführung

    Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse Neurotrophin-3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernatants or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse Neurotrophin-3 standard solution and each sample be measured in duplicate.

    Testpräzision

    • Sample 1: n=16, Mean(pg/ml): 88, Standard deviation: 4.84, CV(%): 5.5
    • Sample 2: n=16, Mean(pg/ml): 329, Standard deviation: 19.74, CV(%): 6
    • Sample 3: n=16, Mean(pg/ml): 682, Standard deviation: 30.01, CV(%): 4.4,
    • Sample 1: n=24, Mean(pg/ml): 113, Standard deviation: 7.12, CV(%): 6.3
    • Sample 2: n=24, Mean(pg/ml): 395, Standard deviation: 28.44, CV(%): 7.2
    • Sample 3: n=24, Mean(pg/ml): 753, Standard deviation: 39.16, CV(%): 5.2

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Handhabung

    Avoid multiple freeze-thaw cycles.

    Lagerung

    -20 °C,4 °C

    Informationen zur Lagerung

    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

    Haltbarkeit

    12 months
  • Wang, Li, Kong, Zhao, Yang, Chen, Yan: "Enhanced expression of neurotrophic factors in the injured spinal cord through vaccination with myelin basic protein-derived peptide pulsed dendritic cells." in: Spine, Vol. 40, Issue 2, pp. 95-101, (2015) (PubMed).

    Zhao, Wang, Peng, Ren, Zhang, Guo, Xu, Lu: "Improvement in nerve regeneration through a decellularized nerve graft by supplementation with bone marrow stromal cells in fibrin." in: Cell transplantation, Vol. 23, Issue 1, pp. 97-110, (2014) (PubMed).

  • Target Alle Neurotrophin 3 (NTF3) ELISA Kits anzeigen

    Neurotrophin 3 (NTF3)

    Andere Bezeichnung

    NTF3

    Hintergrund

    Protein Function: Seems to promote the survival of visceral and proprioceptive sensory neurons.

    Background: Neurotrophin-3(NT-3) is a new member of the nerve growth factor gene family, which plays an important role in the development and maintenance of the vertebrate nervous system.NT-3 and its receptor, called neurotrophic tyrosine kinase receptor type 3(Ntrk3, also called TrkC), are expressed early and throughout embryogenesis. NT-3 is one of five neurotrophin growth factors which shape the development of the nervous system by regulating neuronal survival and differentiation. NT-3 may be one of the central nervous system-derived factors that mediate neural crest(NC) cell proliferation in vivo. NT-3 has been mapped to human chromosome 12p and mouse chromosome 6.

    Synonyms: Neurotrophin-3,NT-3,HDNF,Nerve growth factor 2,NGF-2,Neurotrophic factor,Ntf3,Ntf-3,

    Full Gene Name: Neurotrophin-3

    Cellular Localisation: Secreted.

    Gen-ID

    18205

    UniProt

    P20181

    Pathways

    RTK Signalweg, Neurotrophin Signalübertragung
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