Neurotrophin 3 ELISA Kit

Produktnummer ABIN411339

Produktdetails Neurotrophin 3 ELISA Kit

Target: Neurotrophin 3 (NTF3)

Bindungsspezifität: AA 139-257

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.6-1000 pg/mL

Untere Nachweisgrenze: 15.6 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Neurotrophin-3

Marke: PicoKine™

Proben: Cell Culture Supernatant, Serum

Analytische Methode: Quantitative

Spezifität: Expression system for standard: sf21
Immunogen sequence: Y139-T257

Kreuzreaktivität (Details): There is cross-reactivity with NT-4<2.5 % , and no detectable cross-reactivity with any other cytokine.

Homologie: Bovine,Canine,Chicken,Horse,Monkey,Rabbit

Sensitivität: <2pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: sf21
Immunogen sequence: Y139-T257

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Tissue Specificity: Brain and peripheral tissues.

Plattentyp: Pre-coated

Protokoll: mouse Neurotrophin-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Neurotrophin-3 has been precoated onto 96-well plates. Standards(sf21, Y139-T257) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Neurotrophin-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Neurotrophin-3 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse Neurotrophin-3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernatants or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse Neurotrophin-3 standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 88, Standard deviation: 4.84, CV(%): 5.5
• Sample 2: n=16, Mean(pg/ml): 329, Standard deviation: 19.74, CV(%): 6
• Sample 3: n=16, Mean(pg/ml): 682, Standard deviation: 30.01, CV(%): 4.4,
• Sample 1: n=24, Mean(pg/ml): 113, Standard deviation: 7.12, CV(%): 6.3
• Sample 2: n=24, Mean(pg/ml): 395, Standard deviation: 28.44, CV(%): 7.2
• Sample 3: n=24, Mean(pg/ml): 753, Standard deviation: 39.16, CV(%): 5.2

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Referenzen für Neurotrophin 3 ELISA Kit (ABIN411339)

Wang, Li, Kong, Zhao, Yang, Chen, Yan: "Enhanced expression of neurotrophic factors in the injured spinal cord through vaccination with myelin basic protein-derived peptide pulsed dendritic cells." in: Spine, Vol. 40, Issue 2, pp. 95-101, (2015) (PubMed).

Zhao, Wang, Peng, Ren, Zhang, Guo, Xu, Lu: "Improvement in nerve regeneration through a decellularized nerve graft by supplementation with bone marrow stromal cells in fibrin." in: Cell transplantation, Vol. 23, Issue 1, pp. 97-110, (2014) (PubMed).

Details zu Neurotrophin 3

Target: Neurotrophin 3 (NTF3)

Andere Bezeichnung: NTF3 (NTF3 Produkte)

Hintergrund:

Protein Function: Seems to promote the survival of visceral and proprioceptive sensory neurons.

Background: Neurotrophin-3(NT-3) is a new member of the nerve growth factor gene family, which plays an important role in the development and maintenance of the vertebrate nervous system.NT-3 and its receptor, called neurotrophic tyrosine kinase receptor type 3(Ntrk3, also called TrkC), are expressed early and throughout embryogenesis. NT-3 is one of five neurotrophin growth factors which shape the development of the nervous system by regulating neuronal survival and differentiation. NT-3 may be one of the central nervous system-derived factors that mediate neural crest(NC) cell proliferation in vivo. NT-3 has been mapped to human chromosome 12p and mouse chromosome 6.

Synonyms: Neurotrophin-3,NT-3,HDNF,Nerve growth factor 2,NGF-2,Neurotrophic factor,Ntf3,Ntf-3,

Full Gene Name: Neurotrophin-3

Cellular Localisation: Secreted.

Gen-ID: 18205

UniProt: P20181

Pathways: RTK Signalweg, Neurotrophin Signalübertragung