Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

IL-13 ELISA Kit

IL13 Reaktivität: Maus AA 26-131 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Produktnummer ABIN411312
  • Target Alle IL-13 (IL13) ELISA Kits anzeigen
    IL-13 (IL13) (Interleukin 13 (IL13))
    Bindungsspezifität
    AA 26-131
    Reaktivität
    • 12
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    15.6-1000 pg/mL
    Untere Nachweisgrenze
    15.6 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IL-13
    Marke
    PicoKine™
    Proben
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytische Methode
    Quantitative
    Spezifität
    Expression system for standard: E.coli
    Immunogen sequence: S26-F131
    Kreuzreaktivität (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivität
    <2pg/mL
    Benötigtes Material
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: E.coli
    Immunogen sequence: S26-F131
    Featured
    Zu unserem meistverkauften IL13 ELISA Kit
    Top Product
    Discover our top product IL13 ELISA Kit
  • Applikationshinweise
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Plattentyp
    Pre-coated
    Protokoll
    mouse IL-13 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-13 has been precoated onto 96-well plates. Standards(E.coli, S26-F131) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-13 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-13 amount of sample captured in plate.
    Testdurchführung

    Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse IL-13 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse IL-13 standard solution and each sample be measured in duplicate.

    Testpräzision
    • Sample 1: n=16, Mean(pg/ml): 112, Standard deviation: 6.16, CV(%): 5.5
    • Sample 2: n=16, Mean(pg/ml): 355, Standard deviation: 21.3, CV(%): 6
    • Sample 3: n=16, Mean(pg/ml): 628, Standard deviation: 29.52, CV(%): 4.7,
    • Sample 1: n=24, Mean(pg/ml): 149, Standard deviation: 9.24, CV(%): 6.2
    • Sample 2: n=24, Mean(pg/ml): 417, Standard deviation: 29.61, CV(%): 7.1
    • Sample 3: n=24, Mean(pg/ml): 674, Standard deviation: 39.1, CV(%): 5.8
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    Avoid multiple freeze-thaw cycles.
    Lagerung
    -20 °C,4 °C
    Informationen zur Lagerung
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Haltbarkeit
    12 months
  • Han, Zhang, Tan, Liu, Ma, Ou-Yang, Wu: "EPO modified MSCs can inhibit asthmatic airway remodeling in an animal model." in: Journal of cellular biochemistry, Vol. 119, Issue 1, pp. 1008-1016, (2018) (PubMed).

    Li, Li, Zuo, Ke, Yan, Wen, Zhang, Yang: "T-helper type-2 contact hypersensitivity of Balb/c mice aggravated by dibutyl phthalate via long-term dermal exposure." in: PLoS ONE, Vol. 9, Issue 2, pp. e87887, (2014) (PubMed).

  • Target Alle IL-13 (IL13) ELISA Kits anzeigen
    IL-13 (IL13) (Interleukin 13 (IL13))
    Andere Bezeichnung
    IL13 (IL13 Produkte)
    Synonyme
    IL13 ELISA Kit, IL-13 ELISA Kit, P600 ELISA Kit, Il-13 ELISA Kit, L13 ELISA Kit, interleukin 13 ELISA Kit, IL13 ELISA Kit, Il13 ELISA Kit
    Hintergrund

    Protein Function: Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses (By similarity). .

    Background: Interleukin(IL)-13 is a major inducer of fibrosis in many chronic infectious and autoimmune diseases. Recombinant IL-13 protein inhibits inflammatory cytokine production induced by lipopolysaccharide in human peripheral blood monocytes. Moreover, it synergizes with IL-13 in regulating interferon-gamma synthesis in large granular lymphocytes. Interleukin-13 may be critical in regulating inflammatory and immune responses. IL-13 and IL-4 are two cytokines produced by T helper type 2 cells, mast cells, and basophils. In addition to their physiological roles, these cytokines are also implicated in pathological conditions such as asthma and allergy. The genes encoding human IL-4 and IL-13 are located on segment q23-31 of chromosome 5.4 Recombinant mouse IL-13 is a mixture of Ser26-Phe131 amino acids with the molecular mass of 11.5KDa.

    Synonyms: Interleukin-13,IL-13,T-cell activation protein P600,Il13,Il-13,

    Full Gene Name: Interleukin-13

    Cellular Localisation: Secreted.
    Gen-ID
    16163
    UniProt
    P20109
    Pathways
    JAK-STAT Signalweg, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport
Sie sind hier:
Kundenservice